Development of a novel amperometric solid-contact ion-selective sensor for the post-mortem interval determination

Identifier:  TFM:1147

Handle: https://hdl.handle.net/20.500.11797/TFM1147

Authors:  Saez Bravo, Patricia

Abstract:
This master’s thesis is based on the development of an analytical measurement procedure with amperometric detection using screen-printed sensors to relate vitreous humor ions concentration to post-mortem interval. Novel amperometric solid-contact ion-selective electrodes have been developed to improve all the limitations of potentiometric ion-selective electrodes. Most important limitations of potentiometric sensors are the fixed Nernstian sensitivity depending on the charge of the target ion and the need for calibration before every analysis due to the variability of the standard potential. Moreover, amperometric sensors provide higher sensitivity, stability, reproducibility, sensor durability, faster response and lower detection limit.