Detecció simultània de progesterona i estradiol mitjançant assajos basats en aptàmers: problemes i perspectives.

Identifier:  TFM:885

Handle: https://hdl.handle.net/20.500.11797/TFM885

Authors:  Cardozo, Evelin Florencia

Abstract:
There is a need for rapid and reliable detection of sex hormones such as progesterone and estradiol in a wide variety of samples due to their implications in diagnosis, treatment, and monitoring of different pathologies. This work proposes the simultaneous detection of progesterone and estradiol using a three-step aptamer-based detection method. Different surfaces were analyzed for optimal target immobilization and detection based on a competitive assay. In parallel, the amplification and detection of E28 and P5 aptamers via PCR or RPA in combination with ELONA assay was pursued. Future work will focus on the combination of the two assays.