Simultaneous detection of progesterone and estradiol using aptamer-based assays: problems and prospects.

Entity: Universitat Rovira i Virgili (URV)

Confidenciality: No

Education area(s): Nanociència, Materials i Processos - Tecnologia Química de Frontera 

Title in different languages: Detecció simultània de progesterona i estradiol mitjançant assajos basats en aptàmers: problemes i perspectives.

Abstract: There is a need for rapid and reliable detection of sex hormones such as progesterone and estradiol in a wide variety of samples due to their implications in diagnosis, treatment, and monitoring of different pathologies. This work proposes the simultaneous detection of progesterone and estradiol using a three-step aptamer-based detection method. Different surfaces were analyzed for optimal target immobilization and detection based on a competitive assay. In parallel, the amplification and detection of E28 and P5 aptamers via PCR or RPA in combination with ELONA assay was pursued. Future work will focus on the combination of the two assays.

Subject: Bioquímica i biotecnologia

Academic year: 2020-2021

Language: Anglès

Work's public defense date: 2021-09-10

Subject areas: Biochemistry and biotechnology

Student: Cardozo, Evelin Florencia

Department: Química Analítica i Química Orgànica

Creation date in repository: 2021-11-18

Keywords: Aptamers, Steroids, Multiplexing

Title in original language: Simultaneous detection of progesterone and estradiol using aptamer-based assays: problems and prospects.

Access Rights: info:eu-repo/semantics/openAccess

Project director: O'Sullivan, Ciara ; Svobodová, Markéta