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Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals - imarina:9271525

Autor/es de la URV:Prieto Simón, Beatriz
Autor según el artículo:Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH
Direcció de correo del autor:beatriz.prieto-simon@urv.cat
Identificador del autor:0000-0001-8016-1565
Año de publicación de la revista:2022
Tipo de publicación:Journal Publications
Referencia de l'ítem segons les normes APA:Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH (2022). Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals. Biosensors, 12(7), -. DOI: 10.3390/bios12070480
Referencia al articulo segun fuente origial:Biosensors. 12 (7):
Resumen:Rapid, sensitive, selective and portable virus detection is in high demand globally. How-ever, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we fabricated tuneable nanochannels decorated with target-selective bioreceptors that facilitate the size-selective detection of intact viruses. Detection occurred through selective nanochannel blockage of diffusion of a redox probe, [Fe(CN)6]3/4−, allowing a quantifiable change in the oxidation current before and after analyte binding to the bioreceptor immobilised on the spheres. Our model system involved partial surface passivation of the mono-assembled PS spheres, by silica glancing angle deposition, to confine bioreceptor immobilisation specifically to the channels and improve particle detection sensitivity. Virus detection was first optimised and modelled with biotinylated gold nanoparticles, recognised by streptavidin immobilised on the PS layer, reaching a low limit of detection of 37 particles/mL. Intact, label-free virus detection was demonstrated using MS2 bacteriophage (~23–28 nm), a marker of microbiological contamination, showing an excellent limit of detection of ~1.0 pfu/mL. Tuneable nanochannel geometries constructed directly on sensing electrodes offer label-free, sensitive, and cost-efficient point-of-care biosensing platforms that could be applied for a wide range of viruses.
DOI del artículo:10.3390/bios12070480
Enlace a la fuente original:https://www.mdpi.com/2079-6374/12/7/480
Versión del articulo depositado:info:eu-repo/semantics/publishedVersion
Acceso a la licencia de uso:https://creativecommons.org/licenses/by/3.0/es/
Departamento:Enginyeria Electrònica, Elèctrica i Automàtica
URL Documento de licencia:https://repositori.urv.cat/ca/proteccio-de-dades/
Áreas temáticas:Química
Nanoscience & nanotechnology
Medicine (miscellaneous)
Materiais
Instruments & instrumentation
Instrumentation
Engineering (miscellaneous)
Clinical biochemistry
Ciencias sociales
Ciências biológicas iii
Ciências biológicas i
Chemistry, analytical
Biotecnología
Biotechnology
Biomedical engineering
Astronomia / física
Analytical chemistry
Palabras clave:Virus detection
Surface modification
Electrochemical biosensors
Convective assembly
Colloidal crystal
water
virus detection
pathogens
nanoparticles
convective assembly
colloidal crystal
beads
bacteriophage-ms2
Entidad:Universitat Rovira i Virgili
Fecha de alta del registro:2024-08-31
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