Repositori institucional URV
| Autor/es de la URV: | HERNÁNDEZ OTER, RAMÓN / Riu Rusell, Jordi / Rius Ferrús, Francisco Javier / RIUS RUIZ, FRANCESC XAVIER |
| Autor según el artículo: | Hernández R; Vallés C; Benito AM; Maser WK; Xavier Rius F; Riu J |
| Direcció de correo del autor: | jordi.riu@urv.cat |
| Identificador del autor: | 0000-0001-5823-9223 |
| Año de publicación de la revista: | 2014 |
| Tipo de publicación: | Journal Publications |
| Referencia de l'ítem segons les normes APA: | Hernández R; Vallés C; Benito AM; Maser WK; Xavier Rius F; Riu J (2014). Graphene-based potentiometric biosensor for the immediate detection of living bacteria. Biosensors & Bioelectronics, 54(), 553-557. DOI: 10.1016/j.bios.2013.11.053 |
| Referencia al articulo segun fuente origial: | Biosensors & Bioelectronics. 54 553-557 |
| Resumen: | In this communication we present a potentiometric aptasensor based on chemically modified graphene (transducer layer of the aptasensor) and aptamers (sensing layer). Graphene oxide (GO) and reduced graphene oxide (RGO) are the basis for the construction of two versions of the aptasensor for the detection of a challenging living organism such as Staphylococcus aureus. In these two versions, DNA aptamers are either covalently (in the GO case) or non-covalently (in the RGO case) attached to the transducer layer. In both cases we are able to selectively detect a single CFU/mL of S. aureus in an assay close to real time, although the noise level associated to the aptasensors made with RGO is lower than the ones made with GO. These new aptasensors, that show a high selectivity, are characterized by the simplicity of the technique and the materials used for their construction while offering ultra-low detection limits in very short time responses in the detection of microorganisms. © 2013 Elsevier B.V. |
| DOI del artículo: | 10.1016/j.bios.2013.11.053 |
| Enlace a la fuente original: | https://www.sciencedirect.com/science/article/abs/pii/S0956566313008439 |
| Versión del articulo depositado: | info:eu-repo/semantics/acceptedVersion |
| Acceso a la licencia de uso: | https://creativecommons.org/licenses/by/3.0/es/ |
| Departamento: | Química Analítica i Química Orgànica |
| URL Documento de licencia: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Áreas temáticas: | Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Engenharias iii Engenharias ii Electrochemistry Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Chemistry, analytical Biotecnología Biotechnology & applied microbiology Biotechnology Biophysics Biomedical engineering Biodiversidade Astronomia / física |
| Palabras clave: | Graphene Biosensor Bacteria Aptamer biosensor bacteria aptamer |
| Entidad: | Universitat Rovira i Virgili |
| Fecha de alta del registro: | 2024-10-12 |
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| DocumentPrincipal | DocumentPrincipal | application/pdf |
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