Repositori institucional URV
| Autor/s de la URV: | Guimera Manrique, Roger / Sales Pardo, Marta |
| Autor segons l'article: | Doerrier, Carolina; Gama-Perez, Pau; Pesta, Dominik; Distefano, Giovanna; Soendergaard, Stine D; Chroeis, Karoline Maise; Gonzalez-Franquesa, Alba; Goodpaster, Bret H; Prats, Clara; Sales-Pardo, Marta; Guimera, Roger; Coen, Paul M; Gnaiger, Erich; Larsen, Steen; Garcia-Roves, Pablo M |
| Adreça de correu electrònic de l'autor: | roger.guimera@urv.cat marta.sales@urv.cat |
| Identificador de l'autor: | 0000-0002-3597-4310 0000-0002-8140-6525 |
| Any de publicació de la revista: | 2024 |
| Tipus de publicació: | Journal Publications |
| Referència de l'ítem segons les normes APA: | Doerrier, Carolina; Gama-Perez, Pau; Pesta, Dominik; Distefano, Giovanna; Soendergaard, Stine D; Chroeis, Karoline Maise; Gonzalez-Franquesa, Alba; Go (2024). Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. Free Radical Biology And Medicine, 223(), 384-397. DOI: 10.1016/j.freeradbiomed.2024.07.039 |
| Referència a l'article segons font original: | Free Radical Biology And Medicine. 223 384-397 |
| Resum: | Aim: High-resolution respirometry in human permeabilized muscle fibers is extensively used for analysis of mitochondrial adaptions to nutrition and exercise interventions, and is linked to athletic performance. However, the lack of standardization of experimental conditions limits quantitative inter- and intra-laboratory comparisons. Methods: In our study, an international team of investigators measured mitochondrial respiration of permeabilized muscle fibers obtained from three biopsies ( vastus lateralis) from the same healthy volunteer to avoid inter-individual variability. High-resolution respirometry assays were performed together at the same laboratory to assess whether the heterogenity in published results are due to the effects of respiration media (MiR05 versus Z) with or without the myosin inhibitor blebbistatin at low- and high-oxygen regimes. Results: Our findings reveal significant differences between respiration media for OXPHOS and ETcapacities supported by NADH&succinate-linked substrates at different oxygen concentrations. Respiratory capacities were approximately 1.5-fold higher in MiR05 at high-oxygen regimes compared to medium Z near air saturation. The presence or absence of blebbistatin in human permeabilized muscle fiber preparations was without effect on oxygen flux. Conclusion: Our study constitutes a basis to harmonize and establish optimum experimental conditions for respirometric studies of permeabilized human skeletal muscle fibers to improve reproducibility. |
| DOI de l'article: | 10.1016/j.freeradbiomed.2024.07.039 |
| Enllaç font original: | https://www.sciencedirect.com/science/article/pii/S0891584924005859?dgcid=rss_sd_all |
| Versió de l'article dipositat: | info:eu-repo/semantics/publishedVersion |
| Accès a la llicència d'ús: | https://creativecommons.org/licenses/by/3.0/es/ |
| Departament: | Enginyeria Química |
| URL Document de llicència: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Àrees temàtiques: | Zootecnia / recursos pesqueiros Química Psicología Physiology (medical) Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Endocrinology & metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry Astronomia / física |
| Paraules clau: | Small biopsies Responses Respiration medium z Respiration medium mir05 Phosphorylation Permeabilized skeletal muscle fibers Performance Oxygen consumption Oxygen Oxphos protocols Oxidative phosphorylation O2 regim O(2) regime O regime 2 Muscle fibers, skeletal Mitochondrial respiration Mitochondria, muscle Male Humans Human-cells High-resolution respirometry Heterocyclic compounds, 4 or more rings Healthy volunteers H2o2 emission Exercise Cell respiration Capacit Blebbistatin Adult Adaptations |
| Entitat: | Universitat Rovira i Virgili |
| Data d'alta del registre: | 2024-10-19 |
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| DocumentPrincipal | DocumentPrincipal | application/pdf |
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