Repositori institucional URV
| Autor/es de la URV: | Guimera Manrique, Roger / Sales Pardo, Marta |
| Autor según el artículo: | Doerrier, Carolina; Gama-Perez, Pau; Pesta, Dominik; Distefano, Giovanna; Soendergaard, Stine D; Chroeis, Karoline Maise; Gonzalez-Franquesa, Alba; Goodpaster, Bret H; Prats, Clara; Sales-Pardo, Marta; Guimera, Roger; Coen, Paul M; Gnaiger, Erich; Larsen, Steen; Garcia-Roves, Pablo M |
| Direcció de correo del autor: | roger.guimera@urv.cat marta.sales@urv.cat |
| Identificador del autor: | 0000-0002-3597-4310 0000-0002-8140-6525 |
| Año de publicación de la revista: | 2024 |
| Tipo de publicación: | Journal Publications |
| Referencia de l'ítem segons les normes APA: | Doerrier, Carolina; Gama-Perez, Pau; Pesta, Dominik; Distefano, Giovanna; Soendergaard, Stine D; Chroeis, Karoline Maise; Gonzalez-Franquesa, Alba; Go (2024). Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. Free Radical Biology And Medicine, 223(), 384-397. DOI: 10.1016/j.freeradbiomed.2024.07.039 |
| Referencia al articulo segun fuente origial: | Free Radical Biology And Medicine. 223 384-397 |
| Resumen: | Aim: High-resolution respirometry in human permeabilized muscle fibers is extensively used for analysis of mitochondrial adaptions to nutrition and exercise interventions, and is linked to athletic performance. However, the lack of standardization of experimental conditions limits quantitative inter- and intra-laboratory comparisons. Methods: In our study, an international team of investigators measured mitochondrial respiration of permeabilized muscle fibers obtained from three biopsies ( vastus lateralis) from the same healthy volunteer to avoid inter-individual variability. High-resolution respirometry assays were performed together at the same laboratory to assess whether the heterogenity in published results are due to the effects of respiration media (MiR05 versus Z) with or without the myosin inhibitor blebbistatin at low- and high-oxygen regimes. Results: Our findings reveal significant differences between respiration media for OXPHOS and ETcapacities supported by NADH&succinate-linked substrates at different oxygen concentrations. Respiratory capacities were approximately 1.5-fold higher in MiR05 at high-oxygen regimes compared to medium Z near air saturation. The presence or absence of blebbistatin in human permeabilized muscle fiber preparations was without effect on oxygen flux. Conclusion: Our study constitutes a basis to harmonize and establish optimum experimental conditions for respirometric studies of permeabilized human skeletal muscle fibers to improve reproducibility. |
| DOI del artículo: | 10.1016/j.freeradbiomed.2024.07.039 |
| Enlace a la fuente original: | https://www.sciencedirect.com/science/article/pii/S0891584924005859?dgcid=rss_sd_all |
| Versión del articulo depositado: | info:eu-repo/semantics/publishedVersion |
| Acceso a la licencia de uso: | https://creativecommons.org/licenses/by/3.0/es/ |
| Departamento: | Enginyeria Química |
| URL Documento de licencia: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Áreas temáticas: | Zootecnia / recursos pesqueiros Química Psicología Physiology (medical) Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Endocrinology & metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry Astronomia / física |
| Palabras clave: | Small biopsies Responses Respiration medium z Respiration medium mir05 Phosphorylation Permeabilized skeletal muscle fibers Performance Oxygen consumption Oxygen Oxphos protocols Oxidative phosphorylation O2 regim O(2) regime O regime 2 Muscle fibers, skeletal Mitochondrial respiration Mitochondria, muscle Male Humans Human-cells High-resolution respirometry Heterocyclic compounds, 4 or more rings Healthy volunteers H2o2 emission Exercise Cell respiration Capacit Blebbistatin Adult Adaptations |
| Entidad: | Universitat Rovira i Virgili |
| Fecha de alta del registro: | 2024-10-19 |
| Busca tu registro en: |   |
| Archivo | Descripción | Formato | |
|---|---|---|---|
| DocumentPrincipal | DocumentPrincipal | application/pdf |
© 2011 Universitat Rovira i Virgili