Articles producció científica> Ciències Mèdiques Bàsiques

Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction

  • Dades identificatives

    Identificador: PC:1791
    Handle: https://hdl.handle.net/20.500.11797/PC1791
    Autors:
    Marta TomàsLaura NadalNeus GarciaErica HurtadoAnna SimóMaria A. LanuzaManel SantaféJosep Tomàs
    Resum:
    The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons with different activities (the least active are punished) leads to the loss of roughly half of the overproduced elements and this refines connectivity and increases specificity. The neuromuscular junction is innervated by a single axon at the end of the synapse elimination process and, because of its relative simplicity, has long been used as a model for studying the general principles of synapse development. The involvement of the presynaptic muscarinic ACh autoreceptors may allow for the direct competitive interaction between nerve endings through differential activity-dependent acetylcholine release in the synaptic cleft. Then, the most active ending may directly punish the less active ones. Our previous results indicate the existence in the weakest axons on the polyinnervated neonatal NMJ of an ACh release inhibition mechanism based on mAChR coupled to protein kinase C and voltage-dependent calcium channels. We suggest that this mechanism plays a role in the elimination of redundant neonatal synapses. Results: Here we used confocal microscopy and quantitative morphological analysis to count the number of brightly fluorescent axons per endplate in P7, P9 and P15 transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice. We investigate the involvement of individual mAChR M1-, M2- and M4-subtypes in the control of axonal elimination after the Levator auris longus muscle had been exposed to agonist and antagonist in vivo. We also analysed the role of adenosine receptor subtypes (A1 and A2A) and the tropomyosin-related kinase B receptor. The data sh

  • Altres:

    Autor segons l'article: Marta Tomàs; Laura Nadal; Neus Garcia; Erica Hurtado; Anna Simó; Maria A. Lanuza; Manel Santafé; Josep Tomàs
    Departament: Ciències Mèdiques Bàsiques
    e-ISSN: 1756-6606
    Autor/s de la URV: Tomas Marginet, Marta; Laura Nadal; Neus Garcia; Erica Hurtado; Anna Simó; LANUZA ESCOLANO, MARÍA ANGEL; SANTAFÉ MARTÍNEZ, MANUEL; Josep Tomàs
    Paraules clau: motor nerve terminal motor end-plate cholinergic synapses
    Resum: The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons with different activities (the least active are punished) leads to the loss of roughly half of the overproduced elements and this refines connectivity and increases specificity. The neuromuscular junction is innervated by a single axon at the end of the synapse elimination process and, because of its relative simplicity, has long been used as a model for studying the general principles of synapse development. The involvement of the presynaptic muscarinic ACh autoreceptors may allow for the direct competitive interaction between nerve endings through differential activity-dependent acetylcholine release in the synaptic cleft. Then, the most active ending may directly punish the less active ones. Our previous results indicate the existence in the weakest axons on the polyinnervated neonatal NMJ of an ACh release inhibition mechanism based on mAChR coupled to protein kinase C and voltage-dependent calcium channels. We suggest that this mechanism plays a role in the elimination of redundant neonatal synapses. Results: Here we used confocal microscopy and quantitative morphological analysis to count the number of brightly fluorescent axons per endplate in P7, P9 and P15 transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice. We investigate the involvement of individual mAChR M1-, M2- and M4-subtypes in the control of axonal elimination after the Levator auris longus muscle had been exposed to agonist and antagonist in vivo. We also analysed the role of adenosine receptor subtypes (A1 and A2A) and the tropomyosin-related kinase B receptor. The data show that postnatal axonal elimination is a regulated multireceptor mechanism that guaranteed the monoinnervation of the neuromuscular synapses. Conclusion: The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings, possibly helping to determine the winner or the lossers but, thereafter, the final elimination would occur with some autonomy and independently of postsynaptic maturation.
    Grup de recerca: Unitat d'Histologia i Neurobiologia
    Àrees temàtiques: Health sciences Ciencias de la salud Ciències de la salut
    Accès a la llicència d'ús: thttps://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1756-6606
    Data d'alta del registre: 2016-07-25
    Volum de revista: 9
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Pàgina inicial: 67
    Tipus de publicació: Article Artículo Article

  • Paraules clau:

    Cellular and Molecular Neuroscience; Molecular Biology; Neurosciences
    motor nerve terminal
    motor end-plate
    cholinergic synapses
    Health sciences
    Ciencias de la salud
    Ciències de la salut
    1756-6606

  • Documents:

    DocumentPrincipal

  • Cerca a google

    Search to google scholar