Articles producció científica> Medicina i Cirurgia

Ubiquitous transgenic overexpression of C-C Chemokine Ligand 2: A model to Assess the Combined Effect of High Energy Intake and Continous Low-Grade Inflammation

  • Dades identificatives

    Identificador: PC:586
    Autors:
    Joven, J.Alonso-Villaverde, C.Camps, J.Garcia-Heredia, A.Aragones, G.Sierra-Filardi, E.Corbi, A.Martin Paredero, V.Sirvent, J.Vazquez-Martin, A.Menendez, J.Luciano, F.Beltran, R.Rull, A.Mariné, R.Hernandez-Aguilera, A.Riera, M.Rodriguez-Gallego, E.
    Resum:
    Excessive energy management leads to low-grade, chronic inflammation, which is a significant factor predicting noncommunicable diseases. In turn, inflammation, oxidation, and metabolism are associated with the course of these diseases; mitochondrial dysfunction seems to be at the crossroads of mutual relationships. The migration of immune cells during inflammation is governed by the interaction between chemokines and chemokine receptors. Chemokines, especially C-C-chemokine ligand 2 (CCL2), have a variety of additional functions that are involved in the maintenance of normal metabolism. It is our hypothesis that a ubiquitous and continuous secretion of CCL2 may represent an animal model of low-grade chronic inflammation that, in the presence of an energy surplus, could help to ascertain the afore-mentioned relationships and/or to search for specific therapeutic approaches. Here, we present preliminary data on a mouse model created by using targeted gene knock-in technology to integrate an additional copy of the CCl2 gene in the Gt(ROSA)26Sor locus of the mouse genome via homologous recombination in embryonic stem cells. Short-term dietary manipulations were assessed and the findings include metabolic disturbances, premature death, and the manipulation of macrophage plasticity and autophagy. These results raise a number of mechanistic questions for future study.
  • Altres:

    Autor segons l'article: Joven, J. Alonso-Villaverde, C. Camps, J. Garcia-Heredia, A. Aragones, G. Sierra-Filardi, E. Corbi, A. Martin Paredero, V. Sirvent, J. Vazquez-Martin, A. Menendez, J. Luciano, F. Beltran, R. Rull, A. Mariné, R. Hernandez-Aguilera, A. Riera, M. Rodriguez-Gallego, E.
    Departament: Bioquímica i Biotecnologia Medicina i Cirurgia Ciències Mèdiques Bàsiques
    e-ISSN: 1466-1861
    Autor/s de la URV: Esther Rodríguez-Gallego, Marta Riera-Borrull, Anna Hernández-Aguilera,Roger Mariné-Casadó, Anna Rull, Raúl Beltrán-Debón,Fedra Luciano-Mateo, Javier A. Menendez, Alejandro Vazquez-Martin, Juan J. Sirvent,Vicente Martín-Paredero, Angel L. Corbí, Elena Sierra-Filardi, Gerard Aragonès, Anabel García-Heredia, Jordi Camps, Carlos Alonso-Villaverde, Jorge Joven
    Resum: Excessive energy management leads to low-grade, chronic inflammation, which is a significant factor predicting noncommunicable diseases. In turn, inflammation, oxidation, and metabolism are associated with the course of these diseases; mitochondrial dysfunction seems to be at the crossroads of mutual relationships. The migration of immune cells during inflammation is governed by the interaction between chemokines and chemokine receptors. Chemokines, especially C-C-chemokine ligand 2 (CCL2), have a variety of additional functions that are involved in the maintenance of normal metabolism. It is our hypothesis that a ubiquitous and continuous secretion of CCL2 may represent an animal model of low-grade chronic inflammation that, in the presence of an energy surplus, could help to ascertain the afore-mentioned relationships and/or to search for specific therapeutic approaches. Here, we present preliminary data on a mouse model created by using targeted gene knock-in technology to integrate an additional copy of the CCl2 gene in the Gt(ROSA)26Sor locus of the mouse genome via homologous recombination in embryonic stem cells. Short-term dietary manipulations were assessed and the findings include metabolic disturbances, premature death, and the manipulation of macrophage plasticity and autophagy. These results raise a number of mechanistic questions for future study.
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0962-9351
    Pàgina final: 18
    Volum de revista: 2013
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entitat: Universitat Rovira i Virgili.
    Any de publicació de la revista: 2013
    Pàgina inicial: 1