Autor segons l'article: Pastor A, Rodríguez-Morató J, Olesti E, Pujadas M, Pérez-Mañá C, Khymenets O, Fitó M, Covas MI, Solá R, Motilva MJ, Farré M, de la Torre R
Departament: Medicina i Cirurgia
Autor/s de la URV: Solà Alberich, Rosa Maria
Paraules clau: Virgin olive oil Lc-ms/ms Hydroxytyrosol Humans Health Benzylamine derivatization lc–ms/ms hydroxytyrosol humans health benzylamine derivatization
Resum: Hydroxytyrosol (HT) from olive oil, a potent bioactive molecule with health benefits, has a poor bioavailability, its free form (free HT) being undetectable so far. This fact leads to the controversy whether attained HT concentrations after olive oil polyphenol ingestion are too low to explain the observed biological activities. Due to this, an analytical methodology to determine free HT in plasma is crucial for understanding HT biological activity. Plasma HT instability and low concentrations have been major limitations for its quantification in clinical studies. Here, we describe a method to detect and quantify free HT in human plasma by using liquid chromatography coupled to tandem mass spectrometry. The method encompasses different steps of sample preparation including plasma stabilization, protein precipitation, selective derivatization with benzylamine, and purification by solid-phase extraction. A high sensitivity (LOD, 0.3 ng/mL), specificity and stability of HT is achieved following these procedures. The method was validated and its applicability was demonstrated by analyzing human plasma samples after olive oil intake. A pharmacokinetic comparison was performed measuring free HT plasma concentrations following the intake of 25 mL of ordinary olive oil (nearly undetectable concentrations) versus an extra-virgin olive oil (Cmax = 4.40 ng/mL). To our knowledge, this is the first time that an analytical procedure for quantifying free HT in plasma after olive oil dietary doses has been reported. The present methodology opens the door to a better understanding of the relationship between HT plasma concentrations and its beneficial health effects.
Àrees temàtiques: Zootecnia / recursos pesqueiros Química Organic chemistry Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 00219673
Adreça de correu electrònic de l'autor: rosa.sola@urv.cat
Identificador de l'autor: 0000-0002-8359-235X
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0021967316301029
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Journal Of Chromatography a. 1437 183-190
Referència de l'ítem segons les normes APA: Pastor A, Rodríguez-Morató J, Olesti E, Pujadas M, Pérez-Mañá C, Khymenets O, Fitó M, Covas MI, Solá R, Motilva MJ, Farré M, de la Torre R (2016). Analysis of free hydroxytyrosol in human plasma following the administration of olive oil. Journal Of Chromatography a, 1437(), 183-190. DOI: 10.1016/j.chroma.2016.02.016
DOI de l'article: 10.1016/j.chroma.2016.02.016
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2016
Tipus de publicació: Journal Publications