Articles producció científica> Medicina i Cirurgia

Downregulation of de novo fatty acid synthesis in subcutaneous adipose tissue of moderately obese women

  • Dades identificatives

    Identificador: imarina:3649976
    Autors:
    Guiu-Jurado, EstherAuguet, TeresaBerlanga, AlbaAragones, GemmaAguilar, CarmenSabench, FatimaArmengol, SandraAntonio Porras, JoseMarti, AndreuJorba, RosaHernandez, Mercedel Castillo, DanielRichart, Cristobal
    Resum:
    The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m2, n = 35) and moderately obese women (BMI 30-38 kg/m2, n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity
  • Altres:

    Autor segons l'article: Guiu-Jurado, Esther; Auguet, Teresa; Berlanga, Alba; Aragones, Gemma; Aguilar, Carmen; Sabench, Fatima; Armengol, Sandra; Antonio Porras, Jose; Marti, Andreu; Jorba, Rosa; Hernandez, Merce; del Castillo, Daniel; Richart, Cristobal
    Departament: Bioquímica i Biotecnologia Medicina i Cirurgia
    e-ISSN: 1422-0067
    Autor/s de la URV: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / ARMENGOL LÓPEZ, SANDRA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Jorba Martin, Rosa Maria / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, F
    Paraules clau: Moderate obesity Fatty acid metabolism De novo fatty acid synthesis Adipose tissue fatty acid metabolism de novo fatty acid synthesis adipose tissue
    Resum: The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m2, n = 35) and moderately obese women (BMI 30-38 kg/m2, n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity
    Àrees temàtiques: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14220067
    Adreça de correu electrònic de l'autor: joseantonio.porras@urv.cat danieldel.castillo@urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat rosamaria.jorba@urv.cat
    Identificador de l'autor: 0000-0001-6418-1822 0000-0003-0456-3102 0000-0001-7024-7824 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428 0000-0003-3307-4340
    Pàgina final: 29922
    Data d'alta del registre: 2024-09-28
    Volum de revista: 16
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/1422-0067/16/12/26206
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: International Journal Of Molecular Sciences. 16 (12): 29911-29922
    Referència de l'ítem segons les normes APA: Guiu-Jurado, Esther; Auguet, Teresa; Berlanga, Alba; Aragones, Gemma; Aguilar, Carmen; Sabench, Fatima; Armengol, Sandra; Antonio Porras, Jose; Marti, (2015). Downregulation of de novo fatty acid synthesis in subcutaneous adipose tissue of moderately obese women. International Journal Of Molecular Sciences, 16(12), 29911-29922. DOI: 10.3390/ijms161226206
    DOI de l'article: 10.3390/ijms161226206
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2015
    Pàgina inicial: 29911
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Moderate obesity
    Fatty acid metabolism
    De novo fatty acid synthesis
    Adipose tissue
    fatty acid metabolism
    de novo fatty acid synthesis
    adipose tissue
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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