Autor segons l'article: Levican A; Collado L; Figueras MJ
Departament: Ciències Mèdiques Bàsiques
Autor/s de la URV: Figueras Salvat, María Josefa
Paraules clau: Genetic variability Arcobacter polymerase chain reaction
Resum: The genus Arcobacter includes species considered emerging food and waterborne pathogens. Despite Arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were Arcobacter butzleri and Arcobacter cryaerophilus. This study aimed to establish the prevalence of Arcobacter spp. at a WWTP using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-PCR. In addition, the genetic diversity of the isolates was established using the ERIC-PCR genotyping method. Most of the wastewater samples (96.7%) were positive for Arcobacter and a high genetic diversity was observed among the 651 investigated isolates that belonged to 424 different ERIC genotypes. However, only few strains persisted at different dates or sampling points. The use of direct plating in parallel with culturing after enrichment allowed recovering the species A. butzleri, A. cryaerophilus, Arcobacter thereius, Arcobacter defluvii, Arcobacter skirrowii, Arcobacter ellisii, Arcobacter cloacae, and Arcobacter nitrofigilis, most of them isolated for the first time from wastewater. The predominant species was A. butzleri, however, by direct plating predominated A. cryaerophilus. Therefore, the overall predominance of A. butzleri was a bias associated with the use of enrichment.
Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Geografía General immunology and microbiology General biochemistry,genetics and molecular biology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biotechnology & applied microbiology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física Antropologia / arqueologia
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 23146133
Adreça de correu electrònic de l'autor: mariajose.figueras@urv.cat
Identificador de l'autor: 0000-0002-2268-8980
Data d'alta del registre: 2023-03-04
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.hindawi.com/journals/bmri/2016/8132058/
Referència a l'article segons font original: Biomed Research International. 2016 (8132058): 8132058-
Referència de l'ítem segons les normes APA: Levican A; Collado L; Figueras MJ (2016). The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity of Arcobacter spp. in a Wastewater Treatment Plant. Biomed Research International, 2016(8132058), 8132058-. DOI: 10.1155/2016/8132058
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1155/2016/8132058
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2016
Tipus de publicació: Journal Publications