Autor segons l'article: Ortiz, Mayreli; Torrens, Mabel; Fragoso, Alex; O'Sullivan, Ciara K
Departament: Enginyeria Química
Autor/s de la URV: Fragoso Sierra, Alex / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli / TORRENS DEL VALLE, MABEL
Paraules clau: Base sequence; Celiac disease; Celiac-disease; Colorimetry; Cyclodextrin; Cyclodextrins; Dna detection; Dna probes; Elona; Limit of detection; Oligonucleotides; Polymerase chain reaction; Polymers; Spectrometry, fluorescence; Spectrophotometry, ultraviolet
Resum: In this paper, we describe the development of an enzyme-linked oligonucleotide assay for the detection of a human leukocyte antigen allele associated with celiac disease based on cyclodextrin-modified polymeric surfaces. The surface of maleimide-pre-coated plates was modified with a layer of thiolated cyclodextrin polymer and used for the supramolecular capture of adamantane or ferrocene-modified carboxymethylcellulose polymers bearing DNA probes. The assay was optimised in terms of incubation time, temperature, and surface chemistry and applied to the highly sensitive and selective detection of HLA sequences with a limit of detection of 0.7 nM. A real sample analysed using this platform showed an excellent correlation with maleimide-activated plates using thiolated DNA probes. © 2012 Springer-Verlag.
Àrees temàtiques: Analytical chemistry; Astronomia / física; Biochemical research methods; Biochemistry; Biodiversidade; Biotecnología; Chemistry, analytical; Ciência da computação; Ciência de alimentos; Ciências agrárias i; Ciências ambientais; Ciências biológicas i; Ciências biológicas ii; Ciências biológicas iii; Educação; Educação física; Engenharias i; Engenharias ii; Engenharias iii; Engenharias iv; Ensino; Farmacia; General medicine; Geociências; Interdisciplinar; Materiais; Medicina i; Medicina ii; Medicina iii; Medicina veterinaria; Nutrição; Odontología; Química; Saúde coletiva; Zootecnia / recursos pesqueiros
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: alex.fragoso@urv.cat; mayreli.ortiz@urv.cat; mayreli.ortiz@urv.cat
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://link.springer.com/article/10.1007/s00216-012-5791-3
Referència a l'article segons font original: Analytical And Bioanalytical Chemistry. 403 (1): 195-202
Referència de l'ítem segons les normes APA: Ortiz, Mayreli; Torrens, Mabel; Fragoso, Alex; O'Sullivan, Ciara K (2012). Highly sensitive colorimetric enzyme linked oligonucleotide assay based on cyclodextrin-modified polymeric surfaces. Analytical And Bioanalytical Chemistry, 403(1), 195-202. DOI: 10.1007/s00216-012-5791-3
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1007/s00216-012-5791-3
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2012
Tipus de publicació: Journal Publications
Repositori URV