Autor segons l'article: Ortiz, Mayreli; Fragoso, Alex; Ortiz, Pedro J; O'Sullivan, Ciara K
Departament: Enginyeria Química
Autor/s de la URV: Fragoso Sierra, Alex / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Paraules clau: Synchronous fluorescence; Stern-volmer plot; Nucleic-acids; Molecules; Methylene blue; Intercalation; Guanine bases; G-quadruplexes; Fluorescence; Electrostatic interactions; Dna; Damage; Binding; Base sequence; Aqueous-solution
Resum: The interaction of photosensitizer methylene blue (MB) with single stranded oligonucleotides of composition AAA-AAA-AAA (A(9)), CCC-CCC-CCC (C-9), GGG-GGG-GGG (G(9)), and TTT-TTT-TTT (T-9) has been studied using UV-vis and fluorescence spectroscopies. The quenching effect of single stranded G(9) over MB is favored over the other sequences. The analysis of the fluorescence data by Benesi-Hildebrand plot shows that MB forms 1:1 complex with G(9). Results from spectroscopic experiments, Stern-Volmer and van't Hoff plots are consistent with a principal contribution of the static quenching on the overall quenching mechanism and with the electrostatic binding mode of MB-single stranded G(9) system. A quantum chemical modeling at DFT//B3LYP/6-31G(d,p) level confirms the experimental results. Asides from providing an insight into the underlying mechanism of the interaction between methylene blue and DNA, these results can be exploited for the design of novel DNA sensors using photoactive labels. (C) 2010 Elsevier B.V. All rights reserved.
Àrees temàtiques: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Physics and astronomy (miscellaneous); Physics and astronomy (all); Medicina ii; Materiais; Interdisciplinar; General physics and astronomy; General chemistry; General chemical engineering; Farmacia; Ensino; Engenharias iv; Engenharias iii; Engenharias ii; Engenharias i; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Chemistry, physical; Chemistry (miscellaneous); Chemistry (all); Chemical engineering (miscellaneous); Chemical engineering (all); Biotecnología; Biodiversidade; Astronomia / física; Administração pública e de empresas, ciências contábeis e turismo
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: mayreli.ortiz@urv.cat; mayreli.ortiz@urv.cat; alex.fragoso@urv.cat
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S1010603010004739
Referència a l'article segons font original: Journal Of Photochemistry And Photobiology A-Chemistry. 218 (1): 26-32
Referència de l'ítem segons les normes APA: Ortiz, Mayreli; Fragoso, Alex; Ortiz, Pedro J; O'Sullivan, Ciara K (2011). Elucidation of the mechanism of single-stranded DNA interaction with methylene blue: A spectroscopic approach. Journal Of Photochemistry And Photobiology A-Chemistry, 218(1), 26-32. DOI: 10.1016/j.jphotochem.2010.11.020
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1016/j.jphotochem.2010.11.020
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2011
Tipus de publicació: Journal Publications
Repositori URV