Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells

  • Dades identificatives

    Identificador: imarina:5938157
    Autors:
    Bueno, Maria JJimenez-Renard, VeronicaSamino, SaraCapellades, JordiJunza, AlejandraLuz Lopez-Rodriguez, MariaGarcia-Carceles, JavierLopez-Fabuel, IreneBolanos, Juan PChandel, Navdeep SYanes, OscarColomer, RamonQuintela-Fandino, Miguel
    Resum:
    Upregulation of fatty acid synthase (FASN) is a common event in cancer, although its mechanistic and potential therapeutic roles are not completely understood. In this study, we establish a key role of FASN during transformation. FASN is required for eliciting the anaplerotic shift of the Krebs cycle observed in cancer cells. However, its main role is to consume acetyl-CoA, which unlocks isocitrate dehydrogenase (IDH)-dependent reductive carboxylation, producing the reductive power necessary to quench reactive oxygen species (ROS) originated during the switch from two-dimensional (2D) to three-dimensional (3D) growth (a necessary hallmark of cancer). Upregulation of FASN elicits the 2D-to-3D switch; however, FASN's synthetic product palmitate is dispensable for this process since cells satisfy their fatty acid requirements from the media. In vivo, genetic deletion or pharmacologic inhibition of FASN before oncogenic activation prevents tumor development and invasive growth. These results render FASN as a potential target for cancer prevention studies.
  • Altres:

    Autor segons l'article: Bueno, Maria J; Jimenez-Renard, Veronica; Samino, Sara; Capellades, Jordi; Junza, Alejandra; Luz Lopez-Rodriguez, Maria; Garcia-Carceles, Javier; Lopez-Fabuel, Irene; Bolanos, Juan P; Chandel, Navdeep S; Yanes, Oscar; Colomer, Ramon; Quintela-Fandino, Miguel
    Departament: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/s de la URV: Junza Martínez, Alexandra / SAMINO GENÉ, SARA / Yanes Torrado, Óscar
    Paraules clau: Tumor burden Suppresses Overexpression Orlistat Oncogene Neoplasms, experimental Mice, transgenic Mice, nude Mice, knockout Mice, inbred c57bl Male Inhibition Humans Hek293 cells Glucose-metabolism Fibroblasts Female Fatty acids Fatty acid synthases Expression Embryonic stem cells Embryo, mammalian Cells, cultured Cell line Carboxylase Cancer Animals Activates ppar-alpha
    Resum: Upregulation of fatty acid synthase (FASN) is a common event in cancer, although its mechanistic and potential therapeutic roles are not completely understood. In this study, we establish a key role of FASN during transformation. FASN is required for eliciting the anaplerotic shift of the Krebs cycle observed in cancer cells. However, its main role is to consume acetyl-CoA, which unlocks isocitrate dehydrogenase (IDH)-dependent reductive carboxylation, producing the reductive power necessary to quench reactive oxygen species (ROS) originated during the switch from two-dimensional (2D) to three-dimensional (3D) growth (a necessary hallmark of cancer). Upregulation of FASN elicits the 2D-to-3D switch; however, FASN's synthetic product palmitate is dispensable for this process since cells satisfy their fatty acid requirements from the media. In vivo, genetic deletion or pharmacologic inhibition of FASN before oncogenic activation prevents tumor development and invasive growth. These results render FASN as a potential target for cancer prevention studies.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Physics and astronomy (miscellaneous) Physics and astronomy (all) Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências General physics and astronomy General medicine General chemistry General biochemistry,genetics and molecular biology Farmacia Engenharias iv Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência da computação Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física Antropologia / arqueologia
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20411723
    Adreça de correu electrònic de l'autor: oscar.yanes@urv.cat alexandra.junza@urv.cat
    Identificador de l'autor: 0000-0003-3695-7157 0000-0001-7205-0419
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.nature.com/articles/s41467-019-13028-1
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Nature Communications. 10 (5011): 5011-
    Referència de l'ítem segons les normes APA: Bueno, Maria J; Jimenez-Renard, Veronica; Samino, Sara; Capellades, Jordi; Junza, Alejandra; Luz Lopez-Rodriguez, Maria; Garcia-Carceles, Javier; Lope (2019). Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells. Nature Communications, 10(5011), 5011-. DOI: 10.1038/s41467-019-13028-1
    DOI de l'article: 10.1038/s41467-019-13028-1
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2019
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Chemistry (Miscellaneous),Multidisciplinary Sciences,Physics and Astronomy (Miscellaneous)
    Tumor burden
    Suppresses
    Overexpression
    Orlistat
    Oncogene
    Neoplasms, experimental
    Mice, transgenic
    Mice, nude
    Mice, knockout
    Mice, inbred c57bl
    Male
    Inhibition
    Humans
    Hek293 cells
    Glucose-metabolism
    Fibroblasts
    Female
    Fatty acids
    Fatty acid synthases
    Expression
    Embryonic stem cells
    Embryo, mammalian
    Cells, cultured
    Cell line
    Carboxylase
    Cancer
    Animals
    Activates ppar-alpha
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Planejamento urbano e regional / demografia
    Physics and astronomy (miscellaneous)
    Physics and astronomy (all)
    Odontología
    Nutrição
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geociências
    General physics and astronomy
    General medicine
    General chemistry
    General biochemistry,genetics and molecular biology
    Farmacia
    Engenharias iv
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência da computação
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
    Astronomia / física
    Antropologia / arqueologia
  • Documents:

  • Cerca a google

    Search to google scholar