Articles producció científica> Bioquímica i Biotecnologia

Resveratrol treatment enhances the cellular response to leptin by increasing OBRB content in palmitate-induced steatotic hepg2 cells

  • Dades identificatives

    Identificador: imarina:6030059
    Autors:
    Ardid-Ruiz, AndreaIbars, MariaMena, PedroDel Rio, DanieleMuguerza, BegonaArola, LluisAragones, GerardSuarez, Manuel
    Resum:
    © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The interaction of leptin with its hepatic longest receptor (OBRb) promotes the phosphorylation of signal transducer and activator of transcription-3 (STAT3), protecting the liver from lipid accumulation. However, leptin signalling is disrupted in hepatic steatosis, causing leptin resistance. One promising strategy to combat this problem is the use of bioactive compounds such as polyphenols. Since resveratrol (RSV) is a modulator of lipid homeostasis in the liver, we investigated whether treatment with different doses of RSV restores appropriate leptin action and fat accumulation in palmitate-induced steatotic human hepatoma (HepG2) cells. Both RSV metabolism and the expression of molecules implicated in leptin signalling were analysed. RSV at a 10 μM concentration was entirely metabolized to resveratrol-3-sulfate after 24 and counteracted leptin resistance by increasing the protein levels of OBRb. In addition, RSV downregulated the expression of lipogenic genes including fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) without any significant change in Sirtuin-1 (SIRT1) enzymatic activity. These results demonstrate that RSV restored leptin sensitivity in a cellular model of hepatic steatosis in a SIRT1-independent manner.
  • Altres:

    Autor segons l'article: Ardid-Ruiz, Andrea; Ibars, Maria; Mena, Pedro; Del Rio, Daniele; Muguerza, Begona; Arola, Lluis; Aragones, Gerard; Suarez, Manuel
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Aragonès Bargalló, Gerard / ARDID RUIZ, ANDREA / Arola Ferrer, Luis Maria / IBARS SERRA, MARIA / Muguerza Marquínez, Maria Begoña / Suárez Recio, Manuel
    Paraules clau: Sirtuin 1 Signal transduction Resveratrol metabolites Resveratrol Receptors, leptin Receptor Polyphenols Palmitates Oxidation-reduction Obesity Non-alcoholic fatty liver disease (nafld) Non-alcoholic fatty liver disease Lipogenesis Lipids Lipid metabolism Leptin resistance Leptin Humans Hep g2 cells Gene expression regulation Fatty liver-disease Fatty acids Diet Biomarkers Bioavailability resveratrol metabolites obesity non-alcoholic fatty liver disease (nafld) lipid metabolism leptin resistance
    Resum: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The interaction of leptin with its hepatic longest receptor (OBRb) promotes the phosphorylation of signal transducer and activator of transcription-3 (STAT3), protecting the liver from lipid accumulation. However, leptin signalling is disrupted in hepatic steatosis, causing leptin resistance. One promising strategy to combat this problem is the use of bioactive compounds such as polyphenols. Since resveratrol (RSV) is a modulator of lipid homeostasis in the liver, we investigated whether treatment with different doses of RSV restores appropriate leptin action and fat accumulation in palmitate-induced steatotic human hepatoma (HepG2) cells. Both RSV metabolism and the expression of molecules implicated in leptin signalling were analysed. RSV at a 10 μM concentration was entirely metabolized to resveratrol-3-sulfate after 24 and counteracted leptin resistance by increasing the protein levels of OBRb. In addition, RSV downregulated the expression of lipogenic genes including fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) without any significant change in Sirtuin-1 (SIRT1) enzymatic activity. These results demonstrate that RSV restored leptin sensitivity in a cellular model of hepatic steatosis in a SIRT1-independent manner.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14220067
    Adreça de correu electrònic de l'autor: begona.muguerza@urv.cat lluis.arola@urv.cat gerard.aragones@urv.cat manuel.suarez@urv.cat
    Identificador de l'autor: 0000-0001-7384-8588 0000-0003-2767-1974 0000-0003-0122-8253
    Data d'alta del registre: 2024-06-22
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Referència a l'article segons font original: International Journal Of Molecular Sciences. 20 (24): 6282-
    Referència de l'ítem segons les normes APA: Ardid-Ruiz, Andrea; Ibars, Maria; Mena, Pedro; Del Rio, Daniele; Muguerza, Begona; Arola, Lluis; Aragones, Gerard; Suarez, Manuel (2019). Resveratrol treatment enhances the cellular response to leptin by increasing OBRB content in palmitate-induced steatotic hepg2 cells. International Journal Of Molecular Sciences, 20(24), 6282-. DOI: 10.3390/ijms20246282
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2019
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Sirtuin 1
    Signal transduction
    Resveratrol metabolites
    Resveratrol
    Receptors, leptin
    Receptor
    Polyphenols
    Palmitates
    Oxidation-reduction
    Obesity
    Non-alcoholic fatty liver disease (nafld)
    Non-alcoholic fatty liver disease
    Lipogenesis
    Lipids
    Lipid metabolism
    Leptin resistance
    Leptin
    Humans
    Hep g2 cells
    Gene expression regulation
    Fatty liver-disease
    Fatty acids
    Diet
    Biomarkers
    Bioavailability
    resveratrol metabolites
    obesity
    non-alcoholic fatty liver disease (nafld)
    lipid metabolism
    leptin resistance
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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