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Microscopic imaging of human bloodstains: testing the potential of a confocal laser scanning microscope as an alternative to SEMs

  • Dades identificatives

    Identificador: imarina:6122648
    Autors:
    Hortolà P
    Resum:
    © 2019 Elsevier Ltd The forensic interest on human bloodstains derives from their relation to crime investigation, whereas an archaeological and ethnographic concern arises from their occurrence because of warfare and ritual. The development of digital reflected light microscopes provided an opportunity to use ligh microscopy to study surface topographies in a more accurate way than previously. However, this enhancement has been focused on increasing magnification rather than resolution. An advanced type of light microscope is the confocal laser scanning microscope (CLSM). Its potential as an alternative to scanning electron microscopes (SEMs) for imaging human bloodstains was tested. A fragment of stone (brown chert) was smeared with human peripheral blood, air-dried, and stored indoors. After nearly two years, the sample was examined and imaged using an Olympus LEXT OLS4000 CLSM. The surface detail of CLSM images appeared to be comparatively lower than that of SEM micrographs of coated bloodstains taken at high-vacuum mode and high accelerating voltage, similar to that of SEM micrographs of uncoated bloodstains taken at low-vacuum mode and high accelerating voltage, and similar to or even higher than that of SEM micrographs of uncoated bloodstains taken at high-vacuum mode and low accelerating voltage. These results suggest that a CLSM is a practical alternative to SEMs for imaging human bloodstains when a very-high level of surface detail is not required.
  • Altres:

    Autor segons l'article: Hortolà P
    Departament: Història i Història de l'Art
    Autor/s de la URV: Hortolà i Gómez, Policarp
    Paraules clau: Red blood cells Haemotaphonomy Forensics Ethnography Blood smears Archaeology haemotaphonomy forensics ethnography blood smears archaeology
    Resum: © 2019 Elsevier Ltd The forensic interest on human bloodstains derives from their relation to crime investigation, whereas an archaeological and ethnographic concern arises from their occurrence because of warfare and ritual. The development of digital reflected light microscopes provided an opportunity to use ligh microscopy to study surface topographies in a more accurate way than previously. However, this enhancement has been focused on increasing magnification rather than resolution. An advanced type of light microscope is the confocal laser scanning microscope (CLSM). Its potential as an alternative to scanning electron microscopes (SEMs) for imaging human bloodstains was tested. A fragment of stone (brown chert) was smeared with human peripheral blood, air-dried, and stored indoors. After nearly two years, the sample was examined and imaged using an Olympus LEXT OLS4000 CLSM. The surface detail of CLSM images appeared to be comparatively lower than that of SEM micrographs of coated bloodstains taken at high-vacuum mode and high accelerating voltage, similar to that of SEM micrographs of uncoated bloodstains taken at low-vacuum mode and high accelerating voltage, and similar to or even higher than that of SEM micrographs of uncoated bloodstains taken at high-vacuum mode and low accelerating voltage. These results suggest that a CLSM is a practical alternative to SEMs for imaging human bloodstains when a very-high level of surface detail is not required.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Structural biology Saúde coletiva Química Physics and astronomy (miscellaneous) Physics and astronomy (all) Odontología Nutrição Microscopy Medicina veterinaria Medicina iii Medicina ii Medicina i Materials science (miscellaneous) Materials science (all) Materiais Interdisciplinar Geografía General physics and astronomy General medicine General materials science Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Cell biology Biotecnología Biodiversidade Astronomia / física Antropologia / arqueologia
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: policarp.hortola@urv.cat
    Identificador de l'autor: 0000-0003-2867-6531
    Data d'alta del registre: 2024-02-03
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0968432819303737
    Referència a l'article segons font original: Micron. 130 (UNSP 102821): 102821-
    Referència de l'ítem segons les normes APA: Hortolà P (2020). Microscopic imaging of human bloodstains: testing the potential of a confocal laser scanning microscope as an alternative to SEMs. Micron, 130(UNSP 102821), 102821-. DOI: 10.1016/j.micron.2019.102821
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1016/j.micron.2019.102821
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Cell Biology,Materials Science (Miscellaneous),Microscopy,Physics and Astronomy (Miscellaneous),Structural Biology
    Red blood cells
    Haemotaphonomy
    Forensics
    Ethnography
    Blood smears
    Archaeology
    haemotaphonomy
    forensics
    ethnography
    blood smears
    archaeology
    Zootecnia / recursos pesqueiros
    Structural biology
    Saúde coletiva
    Química
    Physics and astronomy (miscellaneous)
    Physics and astronomy (all)
    Odontología
    Nutrição
    Microscopy
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materials science (miscellaneous)
    Materials science (all)
    Materiais
    Interdisciplinar
    Geografía
    General physics and astronomy
    General medicine
    General materials science
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Cell biology
    Biotecnología
    Biodiversidade
    Astronomia / física
    Antropologia / arqueologia
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