Articles producció científica> Medicina i Cirurgia

Multiplexed monitoring of a novel autoantibody diagnostic signature of colorectal cancer using HaloTag technology-based electrochemical immunosensing platform

  • Dades identificatives

    Identificador: imarina:6157903
    Handle: https://hdl.handle.net/20.500.11797/imarina6157903
    Autors:
    Garranzo-Asensio MGuzmán-Aránguez APovedano EMontiel VRVPoves CFernandez-Aceñero MJMontero-Calle ASolís-Fernández GFernandez-Diez SCamps JArenas MRodríguez-Tomàs EJoven JSanchez-Martinez MRodriguez NDominguez GYáñez-Sedeño PPingarrón JMCampuzano SBarderas R
    Resum:
    © The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. Background and Purpose: The humoral immune response in cancer patients can be used for early detection of the disease. Autoantibodies raised against tumor-associated antigens (TAAs) are promising clinical biomarkers for reliable cancer diagnosis, prognosis, and therapy monitoring. In this study, an electrochemical disposable multiplexed immunosensing platform able to integrate difficult- and easy-to-express colorectal cancer (CRC) TAAs is reported for the sensitive determination of eight CRC-specific autoantibodies. Methods: The electrochemical immunosensing approach involves the use of magnetic microcarriers (MBs) as solid supports modified with covalently immobilized HaloTag fusion proteins for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric responses measured using the hydroquinone (HQ)/H2O2 system were related to the levels of autoantibodies in plasma. Results: The biosensing platform was applied to the analysis of autoantibodies against 8 TAAs described for the first time in this work in plasma samples from healthy asymptomatic individuals (n=3), and patients with high-risk of developing CRC (n=3), and from patients already diagnosed with colorectal (n=3), lung (n=2) or breast (n=2) cancer. The developed bioplatform demonstrated an improved discrimination between CRC patients and controls (asymptomatic healthy individuals and breast and lung cancer patients) compared to an ELISA-like luminescence test. Conclusions: The proposed meth

  • Altres:

    Autor segons l'article: Garranzo-Asensio M; Guzmán-Aránguez A; Povedano E; Montiel VRV; Poves C; Fernandez-Aceñero MJ; Montero-Calle A; Solís-Fernández G; Fernandez-Diez S; Camps J; Arenas M; Rodríguez-Tomàs E; Joven J; Sanchez-Martinez M; Rodriguez N; Dominguez G; Yáñez-Sedeño P; Pingarrón JM; Campuzano S; Barderas R
    Departament: Medicina i Cirurgia Ciències Mèdiques Bàsiques
    Autor/s de la URV: Arenas Prat, Meritxell / Joven Maried, Jorge
    Paraules clau: Salsola-kali pollen Protein Panel Liquid biopsy Identification Humoral immune-response Human serum Halotag fusion proteins Electrochemical bioplatforms Early cancer detection Colorectal cancer Biosensors Biosensor Biomarker determination Antigens halotag fusion proteins electrochemical bioplatforms early cancer detection colorectal cancer biosensor
    Resum: © The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. Background and Purpose: The humoral immune response in cancer patients can be used for early detection of the disease. Autoantibodies raised against tumor-associated antigens (TAAs) are promising clinical biomarkers for reliable cancer diagnosis, prognosis, and therapy monitoring. In this study, an electrochemical disposable multiplexed immunosensing platform able to integrate difficult- and easy-to-express colorectal cancer (CRC) TAAs is reported for the sensitive determination of eight CRC-specific autoantibodies. Methods: The electrochemical immunosensing approach involves the use of magnetic microcarriers (MBs) as solid supports modified with covalently immobilized HaloTag fusion proteins for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric responses measured using the hydroquinone (HQ)/H2O2 system were related to the levels of autoantibodies in plasma. Results: The biosensing platform was applied to the analysis of autoantibodies against 8 TAAs described for the first time in this work in plasma samples from healthy asymptomatic individuals (n=3), and patients with high-risk of developing CRC (n=3), and from patients already diagnosed with colorectal (n=3), lung (n=2) or breast (n=2) cancer. The developed bioplatform demonstrated an improved discrimination between CRC patients and controls (asymptomatic healthy individuals and breast and lung cancer patients) compared to an ELISA-like luminescence test. Conclusions: The proposed methodology uses a just-in-time produced protein in a simpler protocol, with low sample volume, and involves cost-effective instrumentation, which could be used in a high-throughput manner for reliable population screening to facilitate the detection of early CRC patients at affordable cost.
    Àrees temàtiques: Pharmacology, toxicology and pharmaceutics (miscellaneous) Medicine, research & experimental Medicine (miscellaneous)
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 18387640
    Adreça de correu electrònic de l'autor: jorge.joven@urv.cat meritxell.arenas@urv.cat
    Identificador de l'autor: 0000-0003-2749-4541 0000-0003-0815-2570
    Data d'alta del registre: 2023-04-01
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.thno.org/v10p3022.htm
    Referència a l'article segons font original: Theranostics. 10 (7): 3022-3034
    Referència de l'ítem segons les normes APA: Garranzo-Asensio M; Guzmán-Aránguez A; Povedano E; Montiel VRV; Poves C; Fernandez-Aceñero MJ; Montero-Calle A; Solís-Fernández G; Fernandez-Diez S; C (2020). Multiplexed monitoring of a novel autoantibody diagnostic signature of colorectal cancer using HaloTag technology-based electrochemical immunosensing platform. Theranostics, 10(7), 3022-3034. DOI: 10.7150/thno.42507
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.7150/thno.42507
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Tipus de publicació: Journal Publications

  • Paraules clau:

    Medicine (Miscellaneous),Medicine, Research & Experimental,Pharmacology, Toxicology and Pharmaceutics (Miscellaneous)
    Salsola-kali pollen
    Protein
    Panel
    Liquid biopsy
    Identification
    Humoral immune-response
    Human serum
    Halotag fusion proteins
    Electrochemical bioplatforms
    Early cancer detection
    Colorectal cancer
    Biosensors
    Biosensor
    Biomarker determination
    Antigens
    halotag fusion proteins
    electrochemical bioplatforms
    early cancer detection
    colorectal cancer
    biosensor
    Pharmacology, toxicology and pharmaceutics (miscellaneous)
    Medicine, research & experimental
    Medicine (miscellaneous)

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