Autor segons l'article: Baselga-Escudero, Laura; Arola-Arnal, Anna; Pascual-Serrano, Aida; Ribas-Latre, Aleix; Casanova, Ester; Salvado, M-Josepa; Arola, Lluis; Blade, Cinta
Departament: Enginyeria Informàtica i Matemàtiques
Autor/s de la URV: Arola Arnal, Anna / Arola Fernández, Lluís / Arola Ferrer, Luis Maria / BASELGA ESCUDERO, LAURA / BLADÉ SEGARRA, MARIA CINTA / CASANOVA VALLVÉ, ESTER / PASCUAL SERRANO, AÏDA / Ribas Latre, Aleix / Salvadó Rovira, Maria Josepa
Paraules clau: Abca1 protein, rat; Animals; Atherosclerosis; Atp binding cassette transporter 1; Diet; Docosahexaenoic acids; Dyslipidemias; Gene expression regulation; Grape seed extract; Leukocytes, mononuclear; Lipids; Liver; Male; Micrornas; Mirn122 microrna, rat; Mirn33 microrna, rat; Obesity; Organ size; Proanthocyanidins; Rats; Rats, wistar; Rna, messenger; Sterol regulatory element binding protein 2; Zero hunger
Resum: miR-33 and miR-122 are major regulators of lipid metabolism in the liver, and their deregulation has been linked to the development of metabolic diseases such as obesity and metabolic syndrome. However, the biological importance of these miRNAs has been defined using genetic models. The aim of this study was to evaluate whether the levels of miR-122 and miR-33a in rat liver correlate with lipemia in nutritional models. For this purpose, we analyzed the levels of miRNA-33a and miR-122 in the livers of dyslipidemic cafeteria diet-fed rats and of cafeteria diet-fed rats supplemented with proanthocyanidins and/or ω-3 PUFAs because these two dietary components are well-known to counteract dyslipidemia. The results showed that the dyslipidemia induced in rats that were fed a cafeteria diet resulted in the upregulation of miR-33a and miR-122 in the liver, whereas the presence of proanthocyanidins and/or ω-3 PUFAs counteracted the increase of these two miRNAs. However, srebp2, the host gene of miR-33a, was significantly repressed by ω-3 PUFAs but not by proanthocyanidins. Liver mRNA levels of the miR-122 and miR-33a target genes, fas and pparβ/δ, cpt1a and abca1, respectively, were consistent with the expression of these two miRNAs under each condition. Moreover, the miR-33a and abca1 levels were also analyzed in PBMCs. Interestingly, the miR-33a levels evaluated in PBMCs under each condition were similar to the liver levels but enhanced. This demonstrates that miR-33a is expressed in PBMCs and that these cells can be used as a non-invasive way to reflect the expression of this miRNA in the liver. These findings cast new light on the regulation of miR-33a and miR-122 in a dyslipidemic model of obese rats and the way these miRNAs are modulated by dietary components in the liver and in PBMCs. © 2013 Baselga-Escudero et al.
Àrees temàtiques: Administração pública e de empresas, ciências contábeis e turismo; Administração, ciências contábeis e turismo; Agricultural and biological sciences (miscellaneous); Anthropology; Antropologia / arqueologia; Archaeology; Arquitetura, urbanismo e design; Astronomia / física; Biochemistry, genetics and molecular biology (miscellaneous); Biodiversidade; Biology; Biotecnología; Ciência da computação; Ciência de alimentos; Ciência política e relações internacionais; Ciências agrárias i; Ciências ambientais; Ciências biológicas i; Ciências biológicas ii; Ciências biológicas iii; Ciências sociais aplicadas i; Comunicação e informação; Demography; Direito; Economia; Educação; Educação física; Enfermagem; Engenharias i; Engenharias ii; Engenharias iii; Engenharias iv; Ensino; Environmental studies; Farmacia; General agricultural and biological sciences; General biochemistry,genetics and molecular biology; General medicine; Geociências; Geografía; Historia; History & philosophy of science; Human geography and urban studies; Interdisciplinar; Interdisciplinary research in the social sciences; Letras / linguística; Linguística e literatura; Matemática / probabilidade e estatística; Materiais; Medicina i; Medicina ii; Medicina iii; Medicina veterinaria; Medicine (miscellaneous); Multidisciplinary; Multidisciplinary sciences; Nutrição; Odontología; Planejamento urbano e regional / demografia; Psicología; Psychology; Química; Saúde coletiva; Serviço social; Sociología; Sociology; Zootecnia / recursos pesqueiros
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: lluis.arola@urv.cat; anna.arola@urv.cat; lluis.arola@estudiants.urv.cat; lluis.arola@estudiants.urv.cat
ISSN: 19326203
Data d'alta del registre: 2025-01-28
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069817
Referència a l'article segons font original: Plos One. 8 (7): e69817-e69817
Referència de l'ítem segons les normes APA: Baselga-Escudero, Laura; Arola-Arnal, Anna; Pascual-Serrano, Aida; Ribas-Latre, Aleix; Casanova, Ester; Salvado, M-Josepa; Arola, Lluis; Blade, Cinta (2013). Chronic Administration of Proanthocyanidins or Docosahexaenoic Acid Reversess the Increase of miR-33a and miR-122 in Dyslipidemic Obese Rats. Plos One, 8(7), e69817-e69817. DOI: 10.1371/journal.pone.0069817
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1371/journal.pone.0069817
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2013
Tipus de publicació: Journal Publications
Repositori URV