Articles producció científica> Enginyeria Química

Supramolecular Complexes of Plant Neurotoxin Veratridine with Cyclodextrins and Their Antidote-like Effect on Neuro-2a Cell Viability

  • Dades identificatives

    Identificador: imarina:9247754
    Autors:
    Uribe, Laura ALeonardo, SandraNielsen, Thorbjorn TerndrupSteinmann, CasperCampas, MonicaFragoso, Alex
    Resum:
    Veratridine (VTD) is a plant neurotoxin that acts by blocking the voltage-gated sodium channels (VGSC) of cell membranes. Symptoms of VTD intoxication include intense nausea, hypotension, arrhythmia, and loss of consciousness. The treatment for the intoxication is mainly focused on treating the symptoms, meaning there is no specific antidote against VTD. In this pursuit, we were interested in studying the molecular interactions of VTD with cyclodextrins (CDs). CDs are supramolecular macrocycles with the ability to form host–guest inclusion complexes (ICs) inside their hydrophobic cavity. Since VTD is a lipid-soluble alkaloid, we hypothesized that it could form stable inclusion complexes with different types of CDs, resulting in changes to its physicochemical properties. In this investigation, we studied the interaction of VTD with ?-CD, ?-CD and sulfobutyl ether ?-CD (SBCD) by isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR) spectroscopy. Docking and molecular dynamics studies confirmed the most stable configuration for the inclusion complexes. Finally, with an interest in understanding the effects of the VTD/CD molecular interactions, we performed cell-based assays (CBAs) on Neuro-2a cells. Our findings reveal that the use of different amounts of CDs has an antidote-like concentration-dependent effect on the cells, significantly increasing cell viability and thus opening opportunities for novel research on applications of CDs and VTD.
  • Altres:

    Autor segons l'article: Uribe, Laura A; Leonardo, Sandra; Nielsen, Thorbjorn Terndrup; Steinmann, Casper; Campas, Monica; Fragoso, Alex
    Departament: Enginyeria Química
    Autor/s de la URV: CAMPAS HOMS, MONICA / Fragoso Sierra, Alex / Uribe Uribe, Laura Andrea
    Codi de projecte: Grant agreement No. 713679
    Paraules clau: Veratridine Neurotoxin Neuroblastoma 2-a Inclusion complex Cyclodextrin Cellbased assay Cell-based assay Beta-cyclodextrin toxicity solubilization sodium-channels neurotoxin neuroblastoma 2-a molecules inclusion complex glide derivatives cyclodextrin chemistry cell-based assay assay accurate docking
    Resum: Veratridine (VTD) is a plant neurotoxin that acts by blocking the voltage-gated sodium channels (VGSC) of cell membranes. Symptoms of VTD intoxication include intense nausea, hypotension, arrhythmia, and loss of consciousness. The treatment for the intoxication is mainly focused on treating the symptoms, meaning there is no specific antidote against VTD. In this pursuit, we were interested in studying the molecular interactions of VTD with cyclodextrins (CDs). CDs are supramolecular macrocycles with the ability to form host–guest inclusion complexes (ICs) inside their hydrophobic cavity. Since VTD is a lipid-soluble alkaloid, we hypothesized that it could form stable inclusion complexes with different types of CDs, resulting in changes to its physicochemical properties. In this investigation, we studied the interaction of VTD with ?-CD, ?-CD and sulfobutyl ether ?-CD (SBCD) by isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR) spectroscopy. Docking and molecular dynamics studies confirmed the most stable configuration for the inclusion complexes. Finally, with an interest in understanding the effects of the VTD/CD molecular interactions, we performed cell-based assays (CBAs) on Neuro-2a cells. Our findings reveal that the use of different amounts of CDs has an antidote-like concentration-dependent effect on the cells, significantly increasing cell viability and thus opening opportunities for novel research on applications of CDs and VTD.
    Àrees temàtiques: Pharmacology & pharmacy Pharmaceutical science Medicina ii Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: monica.campas@urv.cat lauraandrea.uribe@estudiants.urv.cat lauraandrea.uribe@estudiants.urv.cat alex.fragoso@urv.cat
    Identificador de l'autor: 0000-0002-1220-7100 0000-0003-4885-5393 0000-0003-4885-5393 0000-0003-4839-6094
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/1999-4923/14/3/598
    Programa de finançament: Marie Skłodowska-Curie Actions - European Union's Horizon 2020 research and innovation programme
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Pharmaceutics. 14 (3): 598-
    Referència de l'ítem segons les normes APA: Uribe, Laura A; Leonardo, Sandra; Nielsen, Thorbjorn Terndrup; Steinmann, Casper; Campas, Monica; Fragoso, Alex (2022). Supramolecular Complexes of Plant Neurotoxin Veratridine with Cyclodextrins and Their Antidote-like Effect on Neuro-2a Cell Viability. Pharmaceutics, 14(3), 598-. DOI: 10.3390/pharmaceutics14030598
    Acrònim: MFP
    DOI de l'article: 10.3390/pharmaceutics14030598
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2022
    Acció del programa de finançament: Martí i Franquès COFUND Doctoral Programme
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Pharmaceutical Science,Pharmacology & Pharmacy
    Veratridine
    Neurotoxin
    Neuroblastoma 2-a
    Inclusion complex
    Cyclodextrin
    Cellbased assay
    Cell-based assay
    Beta-cyclodextrin
    toxicity
    solubilization
    sodium-channels
    neurotoxin
    neuroblastoma 2-a
    molecules
    inclusion complex
    glide
    derivatives
    cyclodextrin
    chemistry
    cell-based assay
    assay
    accurate docking
    Pharmacology & pharmacy
    Pharmaceutical science
    Medicina ii
    Farmacia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
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