Autor segons l'article: López M; Blasco L; Gato E; Perez A; Fernández-Garcia L; Martínez-Martinez L; Fernández-Cuenca F; Rodríguez-Baño J; Pascual A; Bou G; Tomás M; Rocío V; Pachón J; Cisneros JM; Garnacho J; Gutierrez-Pizarraya A; Márquez-Vácaro JA; Smani Y; Cano ME; Carmen Fariñas M; Roca I; Espinal P; Sánchez-Porto A; Meruendano GE; Barbeyto-Vales L; Casas-Ciria J; Vallejo L; Fernández-Pérez B; Villar-Chao JC; Padilla-Ortega B; Cercenado-Mansilla E; García-Irure JJ; Jiménez AdA; Gimeno-Cardona C; Valía JC; Tormo-Palop N; Abril V; Rifa J; Martinez-Garcia MJ; Vilaró-Pujals J; Aguirre MN; Vilamala A; Jiménez-Alfaro JA; Reviejo-Jaca C; Casanova PM; Guerreo F; Shaw E; Plasencia V; Nebreda-Mayoral T; Fernández-Calavia MJ; Cruz SGd; Mansilla CA; de Lucas EM; Zorraquino A; Reus-Bañuls S; Garduno-Eseverri E; Sánchez LL; Fleites-Gutiérrez A; Rodríguez-Guardado A; Moreno A; García-Arenzana Anguera JM; Palmero SL; Rodríguez-Maresca M; García-Garrote F; Varela-Otero J; Alonso MdP; Vidal-Verdú E; Rodríguez-López F; Sánchez FP; Ferrer-Vizoso E; Regueiro-Garcia B; Gurgui M; Pericas R; Pomar V; Olaechea-Astigarraga PM; Ayarza-Igartua R; Maciá- Romero MD; de Gopegui-Bordes ER; Sánchez-Romero MI; García-Mata J; Goyanes MJ; Mateos CM; Hernández-Quero J; Escobar-Lara T; Bastardie FB; Iftimie S; Pujol-Bajador I; Galán-Navarro MI; Cádiz-Gurrea ML; Amores-Antequera C; Gómez M; Cantudo P; Martí-Salas C; Cuquet-Peragosa J; Moreno-Flores A; Anibarro-García L; Hernando-Real S; Carrero-González PA; Pallarés-González MA; Fernández SR
Departament: Medicina i Cirurgia
Autor/s de la URV: Iftimie Iftimie, Simona Mihaela
Paraules clau: Type vi secretion Quorum sensing Bile salts Acinetobacter baumannii
Resum: Introduction: Acinetobacter baumannii is an opportunistic nosocomial pathogen associated with multiple infections. This pathogen usually colonizes (first stage of microbial infection) host tissues that are in contact with the external environment. As one of the sites of entry in human hosts is the gastrointestinal tract, the pathogen must be capable of tolerating bile salts. However, studies analyzing the molecular characteristics involved in the response to bile salts in clinical strains of A. baumannii are scarce. Material and Methods: Microbiological and transcriptional studies (arrays and RT-PCR) in the response to bile salts were carried out in isogenic (A. baumanni ΔadeB ATCC 17978 and A. baumannii ΔadeL ATCC 17978) and clinical strains from clone ST79/PFGE-HUI-1 which is characterized by lacking the AdeABC efflux pump and by overexpression the AdeFGH efflux pump. Results and Discussion: In presence of bile salts, in addition to the glutamate/aspartate transporter were found overexpressed in A. baumannii ΔadeB ATCC 17978, the virulence factors (surface motility, biofilm, and Type VI Secretion System) which are associated with activation of the Quorum Sensing system. Overexpression of these factors was confirmed in clinical strains of clone ST79/PFGE-HUI-1. Conclusions: This the first study about the adaptive response to bile salts investigating the molecular and microbiological characteristics in response to bile salts of an isogenic model of A. baumannii ATCC 17978 and clinical isolates of A. baumannii (clinical strains of ST79/PFGE-HUI-1) lacking the main RND efflux pump (AdeABC). Clinical isolates of A. baumannii lacking the AdeABC efflux pump (clone ST79/PFGE-HUI-1) displayed a new clinical profile (increased invasiveness) possibly associated with the response to stress conditions (such as the presence of bile salts).
Àrees temàtiques: Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology Geociências Farmacia Ensino Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: simonamihaela.iftime@urv.cat
Identificador de l'autor: 0000-0003-0714-8414
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Frontiers In Cellular And Infection Microbiology. 7 (MAY): 143-
Referència de l'ítem segons les normes APA: López M; Blasco L; Gato E; Perez A; Fernández-Garcia L; Martínez-Martinez L; Fernández-Cuenca F; Rodríguez-Baño J; Pascual A; Bou G; Tomás M; Rocío V; (2017). Response to bile salts in clinical strains of Acinetobacter baumannii lacking the adeABC efflux pump: Virulence associated with quorum sensing. Frontiers In Cellular And Infection Microbiology, 7(MAY), 143-. DOI: 10.3389/fcimb.2017.00143
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Tipus de publicació: Journal Publications
Repositori URV