Autor segons l'article: Terron-Puig, Margarida; Huber-Ruano, Isabel; Sabadell-Basallote, Joan; Ejarque, Miriam; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Jorba, Rosa; Serena, Carolina; Vendrell, Joan; Fernandez-Veledo, Sonia;
Departament: Medicina i Cirurgia Ciències Mèdiques Bàsiques
Autor/s de la URV: Fernandez Veledo, Sonia / Jorba Martin, Rosa Maria / Maymo Masip, Elsa / Serena Perelló, Carolina / Terrón Puig, Margarida Maria / Vendrell Ortega, Juan José
Paraules clau: Targeting subunits Sirt6 Sirt1 Senescence Pathways Obesity Metabolism Mesenchymal stem-cells Insulin-resistance Inflammation Glycolysis Glycogen Expression Differentiation Aging Adipose-tissue Adipose-derived mesenchymal stromal cells
Resum: Dysfunctional adipocyte precursors have emerged as key determinants for obesity- and aging-related inflammation, but the mechanistic basis remains poorly understood. Here, we explored the dysfunctional adipose tissue of elderly and obese individuals focusing on the metabolic and inflammatory state of human adipose-derived mesenchymal stromal cells (hASCs), and on sirtuins, which link metabolism and inflammation. Both obesity and aging impaired the differentiation potential of hASCs but had a different impact on their proliferative capacity. hASCs from elderly individuals (>= 65 years) showed an upregulation of glycolysis-related genes, which was accompanied by increased lactate secretion and glycogen storage, a phenotype that was exaggerated by obesity. Multiplex protein profiling revealed that the metabolic switch to glycogenesis was associated with a pro-inflammatory secretome concomitant with a decrease in the protein expression of SIRT1 and SIRT6. siRNA-mediated knockdown of SIRT1 and SIRT6 in hASCs from lean adults increased the expression of pro-inflammatory and glycolysis-related markers, and enforced glycogen deposition by overexpression of protein targeting to glycogen (PTG) led to a downregulation of SIRT1/6 protein levels, mimicking the inflammatory state of hASCs from elderly subjects. Overall, our data point to a glycogen-SIRT1/6 signaling axis as a driver of age-related inflammation in adipocyte precursors.
Àrees temàtiques: Medicina veterinaria Geriatrics & gerontology Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Aging
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: carolina.serena@urv.cat elsa.maymo@urv.cat margaridamaria.terron@estudiants.urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat rosamaria.jorba@urv.cat
Identificador de l'autor: 0000-0002-9133-3120 0000-0003-2906-3788 0000-0002-6994-6115 0000-0003-3307-4340
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Aging Cell. 21 (8):
Referència de l'ítem segons les normes APA: Terron-Puig, Margarida; Huber-Ruano, Isabel; Sabadell-Basallote, Joan; Ejarque, Miriam; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Jorba, Rosa; Serena, C (2022). Glycogen accumulation in adipocyte precursors from elderly and obese subjects triggers inflammation via SIRT1/6 signaling. Aging Cell, 21(8), -. DOI: 10.1111/acel.13667
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV