Articles producció científica> Enginyeria Química

Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection

  • Dades identificatives

    Identificador: imarina:9294255
    Autors:
    Torregrosa, DanielJauset-Rubio, MiriamSerrano, RaquelSvobodov, MarketaGrindlay, GuillermoO'Sullivan, Ciara KMora, Juan
    Resum:
    The overall objective of this work is the evaluation of different competitive aptamer assays based on inductively coupled plasma mass spectrometry (ICP-MS) detection for the determination of β-conglutin (food protein allergen from lupin) in flour samples. To this end, two competitive aptamer assay schemes were developed using either thiolated aptamers chemisorbed onto gold nanoparticles (AuNPs) or biotinylated aptamers linked to streptavidin-AuNPs. The influence of ICP-MS detection mode (i.e., conventional vs single particle) on assay performance was explored. In the case of the thiolated aptamer, the limit of detection (LoD) obtained using the single particle mode was improved 2-fold as compared to the LoD provided by the conventional mode. With regards to the biotinylated aptamer, the use of the conventional mode provided a 5-fold improvement of LoD as compared to that obtained for the single particle one. Using the optimized conditions, the best LoD of 2 pM was obtained with the biotinylated aptamer operating with conventional ICP-MS detection. When compared to previous reports using the same aptamer in a competitive assay, the developed method significantly improved the LoD by at least an order of magnitude. Different flour samples containing lupin were successfully analyzed according to European Conformity guidelines for the analysis of food contaminants.Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
  • Altres:

    Autor segons l'article: Torregrosa, Daniel; Jauset-Rubio, Miriam; Serrano, Raquel; Svobodov, Marketa; Grindlay, Guillermo; O'Sullivan, Ciara K; Mora, Juan
    Departament: Enginyeria Química
    Autor/s de la URV: Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / SVOBODOVÁ, MARKÉTA
    Paraules clau: Β-conglutin Single particle Seed storage proteins Nanoparticles Metal nanoparticles Mass spectrometry Lupinus Inductively coupled plasma mass spectrometry Gold Aptamers, nucleotide Aptamer assay Allergens
    Resum: The overall objective of this work is the evaluation of different competitive aptamer assays based on inductively coupled plasma mass spectrometry (ICP-MS) detection for the determination of β-conglutin (food protein allergen from lupin) in flour samples. To this end, two competitive aptamer assay schemes were developed using either thiolated aptamers chemisorbed onto gold nanoparticles (AuNPs) or biotinylated aptamers linked to streptavidin-AuNPs. The influence of ICP-MS detection mode (i.e., conventional vs single particle) on assay performance was explored. In the case of the thiolated aptamer, the limit of detection (LoD) obtained using the single particle mode was improved 2-fold as compared to the LoD provided by the conventional mode. With regards to the biotinylated aptamer, the use of the conventional mode provided a 5-fold improvement of LoD as compared to that obtained for the single particle one. Using the optimized conditions, the best LoD of 2 pM was obtained with the biotinylated aptamer operating with conventional ICP-MS detection. When compared to previous reports using the same aptamer in a competitive assay, the developed method significantly improved the LoD by at least an order of magnitude. Different flour samples containing lupin were successfully analyzed according to European Conformity guidelines for the analysis of food contaminants.Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
    Àrees temàtiques: Spectroscopy Química Odontología Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências General medicine Farmacia Environmental chemistry Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Astronomia / física Analytical chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: miriam.jauset@urv.cat
    Identificador de l'autor: 0000-0002-9943-6132
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/pii/S0003267023002635
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Analytica Chimica Acta. 1252 341042-341042
    Referència de l'ítem segons les normes APA: Torregrosa, Daniel; Jauset-Rubio, Miriam; Serrano, Raquel; Svobodov, Marketa; Grindlay, Guillermo; O'Sullivan, Ciara K; Mora, Juan (2023). Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection. Analytica Chimica Acta, 1252(), 341042-341042. DOI: 10.1016/j.aca.2023.341042
    DOI de l'article: 10.1016/j.aca.2023.341042
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Analytical Chemistry,Biochemistry,Chemistry, Analytical,Environmental Chemistry,Spectroscopy
    Β-conglutin
    Single particle
    Seed storage proteins
    Nanoparticles
    Metal nanoparticles
    Mass spectrometry
    Lupinus
    Inductively coupled plasma mass spectrometry
    Gold
    Aptamers, nucleotide
    Aptamer assay
    Allergens
    Spectroscopy
    Química
    Odontología
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Environmental chemistry
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Biochemistry
    Astronomia / física
    Analytical chemistry
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