Molecular Adaptations of BDNF/NT-4 Neurotrophic and Muscarinic Pathways in Ageing Neuromuscular Synapses

Autor segons l'article: Balanya-Segura, Marta; Polishchuk, Aleksandra; Just-Borras, Laia; Cilleros-Mane, Victor; Silvera, Carolina; Ardevol, Anna; Tomas, Marta; Lanuza, Maria A; Hurtado, Erica; Tomas, Josep

Departament: Ciències Mèdiques Bàsiques

Autor/s de la URV: Ardévol Grau, Anna / Balanya Segura, Marta / Cilleros Mañé, Víctor / Hurtado Caballero, Erica / Just Borràs, Laia / Lanuza Escolano, María Angel / POLISHCHUK, ALEKSANDRA / Tomás Ferré, José Maria / Tomas Marginet, Marta / Tomàs Porres, Josep

Paraules clau: Acetylcholine release; Acetylcholine-release; Aging; Animals; B receptor; Bdnf; Bdnf protein, rat; Brain-derived neurotrophic factor; Dependent phosphorylation; Down-regulation; Functional interactions; Male; Motor endplate; Muscarinic receptors; Nerve growth factors; Neuromuscular junction; Neuronal responses; Neurotrophin 4; Protein-kinase-c; Rats; Rats, wistar; Receptor, trkb; Receptors, muscarinic; Receptors, nerve growth factor; Serine kinases; Signal transduction; Skeletal-muscle cells; Soluble n-ethylmaleimide-sensitive fusion protein attachment protein receptor (snare) comple; Soluble n-ethylmaleimide-sensitive fusion protein attachment protein receptor (snare) complex; Synaptic transmission; Transmitter release; Trkb

Resum: Age-related conditions, such as sarcopenia, cause physical disabilities for an increasing section of society. At the neuromuscular junction, the postsynaptic-derived neurotrophic factors brain-derived neurotrophic factor (BDNF) and neurotrophin 4 (NT-4) have neuroprotective functions and contribute to the correct regulation of the exocytotic machinery. Similarly, presynaptic muscarinic signalling plays a fundamental modulatory function in this synapse. However, whether or not these signalling pathways are compromised in ageing neuromuscular system has not yet been analysed. The present study analyses, through Western blotting, the differences in expression and activation of the main key proteins of the BDNF/NT-4 and muscarinic pathways related to neurotransmission in young versus ageing Extensor digitorum longus (EDL) rat muscles. The main results show an imbalance in several sections of these pathways: (i) a change in the stoichiometry of BDNF/NT-4, (ii) an imbalance of Tropomyosin-related kinase B receptor (TrkB)-FL/TrkB-T1 and neurotrophic receptor p 75 (p75NTR), (iii) no changes in the cytosol/membrane distribution of phosphorylated downstream protein kinase C (PKC)beta I and PKC epsilon, (iv) a reduction in the M2-subtype muscarinic receptor and P/Q-subtype voltage-gated calcium channel, (v) an imbalance of phosphorylated mammalian uncoordinated-18-1 (Munc18-1) (S313) and synaptosomal-associated protein 25 (SNAP-25) (S187), and (vi) normal levels of molecules related to the management of acetylcholine (Ach). Based on this descriptive analysis, we hypothesise that these pathways can be adjusted to ensure neurotransmission rather than undergoing negative alterations caused by ageing. However, further studies are needed to assess this hypothetical suggestion. Our results contribute to the understanding of some previously described neuromuscular functional age-related impairments. Strategies to promote these signalling pathways could improve the neuromuscular physiology and quality of life of older people.

Grup de recerca: Unitat d'Histologia i Neurobiologia

Àrees temàtiques: Astronomia / física; Biochemistry & molecular biology; Biodiversidade; Biotecnología; Catalysis; Chemistry, multidisciplinary; Ciência da computação; Ciência de alimentos; Ciências agrárias i; Ciências ambientais; Ciências biológicas i; Ciências biológicas ii; Ciências biológicas iii; Computer science applications; Educação física; Engenharias i; Engenharias ii; Engenharias iv; Farmacia; Geociências; Inorganic chemistry; Interdisciplinar; Materiais; Medicina i; Medicina ii; Medicina iii; Medicina veterinaria; Medicine (miscellaneous); Molecular biology; Nutrição; Odontología; Organic chemistry; Physical and theoretical chemistry; Psicología; Química; Saúde coletiva; Spectroscopy; Zootecnia / recursos pesqueiros

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

Adreça de correu electrònic de l'autor: mariaangel.lanuza@urv.cat; laia.just@urv.cat; josepmaria.tomas@urv.cat; victor.cilleros@alumni.urv.cat; anna.ardevol@urv.cat; marta.balanya@urv.cat; aleksandra.polishchuk@urv.cat; josep.tomaspo@estudiants.urv.cat; erica.hurtado@urv.cat; aleksandra.polishchuk@urv.cat; marta.tomas@urv.cat; laia.just@urv.cat; marta.balanya@urv.cat

Data d'alta del registre: 2024-12-28

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Referència a l'article segons font original: International Journal Of Molecular Sciences. 25 (15): 8018-

Referència de l'ítem segons les normes APA: Balanya-Segura, Marta; Polishchuk, Aleksandra; Just-Borras, Laia; Cilleros-Mane, Victor; Silvera, Carolina; Ardevol, Anna; Tomas, Marta; Lanuza, Maria (2024). Molecular Adaptations of BDNF/NT-4 Neurotrophic and Muscarinic Pathways in Ageing Neuromuscular Synapses. International Journal Of Molecular Sciences, 25(15), 8018-. DOI: 10.3390/ijms25158018

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

DOI de l'article: 10.3390/ijms25158018

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2024

Tipus de publicació: Journal Publications