Genomic Sequencing and Functional Analysis of the Ex-Type Strain of Malbranchea zuffiana.

Autor segons l'article: Granados-Casas AO; Fernández-Bravo A; Stchigel AM; Cano-Lira JF

Departament: Ciències Mèdiques Bàsiques

Autor/s de la URV: Cano Lira, José Francisco / Fernández Bravo, Ana / Granados Casas, Alan Omar / Stchigel Glikman, Alberto Miguel

Paraules clau: Proteins; Phylogenomics; Onygenales; Malbranchea zuffiana; Keratin degradation; Genome assembly; Genes

Resum: Malbranchea is a genus within the order Onygenales (phylum Ascomycota) that includes predominantly saprobic cosmopolitan species. Despite its ability to produce diverse secondary metabolites, no genomic data for Malbranchea spp. are currently available in databases. Therefore, in this study, we obtained, assembled, and annotated the genomic sequence of the ex-type strain of Malbranchea zuffiana (CBS 219.58). For the genomic sequencing, we employed both the Illumina and PacBio platforms, followed by hybrid assembly using MaSuRCA. Quality assessment of the assembly was performed using QUAST and BUSCO tools. Annotation was conducted using BRAKER2, and functional annotation was completed with InterProScan. The resulting genome was of high quality, with a size of 26.46 Mbp distributed across 38 contigs and a BUSCO completion rate of 95.7%, indicating excellent contiguity and assembly completeness. A total of 8248 protein-encoding genes were predicted, with functional annotations assigned to 73.9% of them. Moreover, 82 genes displayed homology with entries in the Pathogen Host Interactions (PHI) database, while 494 genes exhibited similarity to entries in the Carbohydrate-Active Enzymes (CAZymes) database. Furthermore, 30 biosynthetic gene clusters (BGCs) were identified, suggesting significant potential for the biosynthesis of diverse secondary metabolites. Comparative functional analysis with closely related species unveiled a considerable abundance of domains linked to enzymes involved in keratin degradation, alongside a restricted number of domains associated with enzymes engaged in plant cell wall degradation in all studied species of the Onygenales. This genome-based elucidation not only enhances our comprehension of the biological characteristics of M. zuffiana but also furnishes valuable insights for subsequent investigations concerning Malbranchea species and the order Onygenales.

Àrees temàtiques: Plant science; Mycology; Microbiology (medical); Microbiology; Farmacia; Ecology, evolution, behavior and systematics; Ciências biológicas iii; Ciências biológicas ii

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

Adreça de correu electrònic de l'autor: alanomar.granados@urv.cat; ana.fernandez@urv.cat; alanomar.granados@urv.cat; albertomiguel.stchigel@urv.cat; jose.cano@urv.cat

Data d'alta del registre: 2024-10-05

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.mdpi.com/2309-608X/10/9/600

Referència a l'article segons font original: J Fungi (Basel). 10 (9): 600-

Referència de l'ítem segons les normes APA: Granados-Casas AO; Fernández-Bravo A; Stchigel AM; Cano-Lira JF (2024). Genomic Sequencing and Functional Analysis of the Ex-Type Strain of Malbranchea zuffiana.. J Fungi (Basel), 10(9), 600-. DOI: 10.3390/jof10090600

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

DOI de l'article: 10.3390/jof10090600

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2024

Tipus de publicació: Journal Publications