Depuration of Aliarcobacter butzleri and Malaciobacter molluscorum in Comparison with Escherichia coli in Mussels (Mytilus galloprovincialis) and Oysters (Crassostrea gigas)

Autor segons l'article: Salas-Masso, Nuria; Fernandez-Bravo, Ana; Bertomeu, Edgar; Andree, Karl B; Figueras, Maria Jose; Furones, Dolors

Departament: Ciències Mèdiques Bàsiques

Autor/s de la URV: Fernández Bravo, Ana / Figueras Salvat, María Josefa

Paraules clau: Wate; Vibrio-parahaemolyticus; Temperature; Shellfish; Seasons; Recovery; Pacific oysters; Oyster; Oyste; Mytilus; Mussel; M alaciobacter; Indicator; Escherichia coli; Depuration; Crassostrea; Bivalvia; Arcobacter spp.; Arcobacter; Animals; Aliarcobacter; <italic>m</italic> <italic>alaciobacter</italic>; <italic>escherichia coli</italic>; <italic>aliarcobacter</italic>

Resum: Arcobacter-related species are considered emerging food-borne and waterborne pathogens, with shellfish being a suggested reservoir. In a published study that investigated 204 shellfish samples and 476 isolates, the species Arcobacter butzleri (now known as Aliarcobacter butzleri) and Arcobacter molluscorum (now known as Malaciobacter molluscorum) have been isolated as the most dominant species. However, the efficiency of depuration for eliminating A. butzleri and M. molluscorum in comparison with Escherichia coli from mussels and oysters is unknown and is therefore the objective of this investigation. The shellfish depuration process was evaluated in the laboratory, in summer and winter, using mussels and oysters collected from the Ebro Delta harvesting areas after performing a natural contamination and an artificial contamination using the same conditions for both mollusk and seasons. The natural contamination was performed by exposing the shellfish to a freshwater channel that receives untreated sewage from the village of Poble Nou (PNC) and that had a salinity of 10.7-16.8 parts per thousand. The artificial contamination exposed the shellfish to A. butzleri and E. coli (in one tank) and to M. molluscorum and E. coli in another tank under controlled conditions of salinity (34.5 parts per thousand) and temperature (20 degrees C summer and 14 degrees C winter). When evaluating the reduction in the bacteria load (every 24 h) throughout 120 h, the naturally contaminated shellfish at the PNC showed a higher reduction than the shellfish contaminated at the laboratory, with the exception of M. molluscorum, that at 24 h could not be detected in summer, neither in mussels nor in oysters. This may be attributed to the fact that the bacteria from the PNC were less adapted to the conditions of high salinity (34.5 parts per thousand) in which the depuration process was performed. Although temperature did not statistically make a difference in depuration, at 20 degrees C a higher elimination of all bacteria was recorded relative to 14 degrees C. In general, E. coli survived more in mussels than in oysters, and M. molluscorum suffered in both mollusks a higher reduction than A. butzleri. New studies are required to determine further the safety of bivalves regarding the presence of Arcobacter-related species.

Àrees temàtiques: Molecular biology; Microbiology (medical); Microbiology; Infectious diseases; Immunology and microbiology (miscellaneous); Immunology and microbiology (all); Immunology and allergy; General immunology and microbiology; Farmacia

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

Adreça de correu electrònic de l'autor: ana.fernandez@urv.cat; mariajose.figueras@urv.cat

Data d'alta del registre: 2025-03-22

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.mdpi.com/2076-0817/13/11/973

Referència a l'article segons font original: Pathogens. 13 (11): 973-

Referència de l'ítem segons les normes APA: Salas-Masso, Nuria; Fernandez-Bravo, Ana; Bertomeu, Edgar; Andree, Karl B; Figueras, Maria Jose; Furones, Dolors (2024). Depuration of Aliarcobacter butzleri and Malaciobacter molluscorum in Comparison with Escherichia coli in Mussels (Mytilus galloprovincialis) and Oysters (Crassostrea gigas). Pathogens, 13(11), 973-. DOI: 10.3390/pathogens13110973

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

DOI de l'article: 10.3390/pathogens13110973

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2024

Tipus de publicació: Journal Publications