Autor segons l'article: Maside, Carolina; Recuero, Sandra; Salas-Huetos, Albert; Ribas-Maynou, Jordi; Yeste, Marc
Departament: Ciències Mèdiques Bàsiques
Autor/s de la URV: Salas Huetos, Albert
Paraules clau: Tunel assay; Swine; Storag; Spermatozoa; Sperm viability; Sperm viabilit; Sperm motility; Sperm dna fragmentation; Semen analysis; Semen; Reduced glutathione; Motility; Morphometric subpopulations; Morphology; Male; Laboratories; Human spermatozoa; Flow cytometry; Fertilizing-capacity; Farms; Dna integrity; Dna; Chromatin-structure assay; Chromatin; Artificial-insemination; Animals
Resum: Pig breeding is mainly conducted through artificial insemination with liquid-stored semen. It is, there-fore, crucial to ensure that sperm quality is over the standard thresholds, as reduced sperm motility, mor-phology or plasma membrane integrity are associated with reduced farrowing rates and litter sizes. This work aims to summarise the methods utilised in farms and research laboratories to evaluate sperm qual-ity in pigs. The conventional spermiogram consists in the assessment of sperm concentration, motility and morphology, which are the most estimated variables in farms. Yet, while the determination of these sperm parameters is enough for farms to prepare seminal doses, other tests, usually carried out in spe-cialised laboratories, may be required when boar studs exhibit a decreased reproductive performance. These methods include the evaluation of functional sperm parameters, such as plasma membrane integ-rity and fluidity, intracellular levels of calcium and reactive oxygen species, mitochondrial activity, and acrosome integrity, using fluorescent probes and flow cytometry. Furthermore, sperm chromatin conden-sation and DNA integrity, despite not being routinely assessed, may also help determine the causes of reduced fertilising capacity. Sperm DNA integrity can be evaluated through direct (Comet, transferase deoxynucleotide nick end labelling (TUNEL) and its in situ nick variant) or indirect tests (Sperm Chromatin Structure Assay, Sperm Chromatin Dispersion Test), whereas chromatin condensation can be determined with Chromomycin A3. Considering the high degree of chromatin packaging in pig sperm, which only have protamine 1, growing evidence suggests that complete decondensation of that chro-matin is needed before DNA fragmentation through TUNEL or Comet can be examined.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of The Animal Consortium. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Àrees temàtiques: Zootecnia / recursos pesqueiros; Veterinary sciences; Planejamento urbano e regional / demografia; Odontología; Nutrição; Medicina veterinaria; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Interdisciplinar; Geografía; Engenharias iii; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Biotecnología; Biodiversidade; Animal science and zoology; Agriculture, dairy & animal science
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: albert.salas@urv.cat
Data d'alta del registre: 2025-01-08
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S1751731123000162
Referència a l'article segons font original: Animal. 17 (3): 100720-
Referència de l'ítem segons les normes APA: Maside, Carolina; Recuero, Sandra; Salas-Huetos, Albert; Ribas-Maynou, Jordi; Yeste, Marc (2023). Animal board invited review: An update on the methods for semen quality evaluation in swine - from farm to the lab. Animal, 17(3), 100720-. DOI: 10.1016/j.animal.2023.100720
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1016/j.animal.2023.100720
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2023
Tipus de publicació: Journal Publications
Repositori URV