Author, as appears in the article.: Ramon A. Alvarez-Puebla; Nicolas Pazos-Perez; Elena Pazos; Carme Catala; Bernat Mir-Simon; Sara Gómez-de Pedro; Juan Sagales; Carlos Villanueva; Jordi Vila; Alex Soriano; F. Javier García de Abajo
Department: Química Física i Inorgànica
URV's Author/s: ALVAREZ PUEBLA, RAMON ANGEL; Nicolas Pazos-Perez; Elena Pazos; Carme Catala; Bernat Mir-Simon; Sara Gómez-de Pedro; Juan Sagales; Carlos Villanueva; Jordi Vila; Alex Soriano; F. Javier García de Abajo
Keywords: Està en blanc
Abstract: Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (∼1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (∼1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.
Research group: Grupo de Plasmonica y Ultradetección
Thematic Areas: Chemistry Química Química
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2045-2322
Author identifier: 0000-0003-4770-5756; 0000-0002-2326-4231; 0000-0001-9397-2703; n/a; n/a; n/a; n/a; n/a; 0000-0002-8025-3926; n/a; 0000-0002-4970-4565
Record's date: 2016-07-18
Journal volume: 6
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.nature.com/articles/srep29014
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1038/srep29014
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: Art.num. 29014
Publication Type: Article Artículo Article
Repositori URV