Articles producció científica> Ciències Mèdiques Bàsiques

Schizophyllum radiatum, an emerging fungus from human respiratory tract

  • Identification data

    Identifier: PC:1857
    Authors:
    J. GenéJ. P. Z. SiqueiraD. SuttonD. GarcíaM. Guevara-SuarezC. DecockN. WiederholdJ. Guarro
    Abstract:
    Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.
  • Others:

    Author, as appears in the article.: J. Gené; J. P. Z. Siqueira; D. Sutton; D. García; M. Guevara-Suarez; C. Decock; N. Wiederhold; J. Guarro
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: GENÉ DÍAZ, JOSEPA; J. P. Z. Siqueira; D. Sutton; GARCÍA SÁNCHEZ, DANIA; M. Guevara-Suarez; C. Decock; N. Wiederhold; GUARRO ARTIGAS, JOSEP
    Keywords: Està en blanc
    Abstract: Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.
    Research group: Unitat de Micologia i Microbiologia Ambiental
    Thematic Areas: Health sciences Ciencias de la salud Ciències de la salut
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0095-1137
    Author identifier: N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D
    Record's date: 2016-10-28
    Last page: 2497
    Journal volume: 54
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://jcm.asm.org/content/54/10/2491.short
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1128/JCM.01170-16
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    First page: 2491
    Publication Type: Article Artículo Article
  • Keywords:

    Aparell respiratori
    Fongs patògens
    Està en blanc
    Health sciences
    Ciencias de la salud
    Ciències de la salut
    0095-1137
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