Articles producció científica> Bioquímica i Biotecnologia

Saccharomyces and non-Saccharomyces competition during microvinification under different sugar and nitrogen conditions

  • Identification data

    Identifier: PC:2541
    Authors:
    Albert MasJessica LleixàMaria ManzanoMaría del C. Portillo
    Abstract:
    The inoculation of wines with autochthonous yeast allows obtaining complex wines with a peculiar microbial footprint characteristic from a wine region. Mixed inoculation of non-Saccharomyces yeasts and S. cerevisiae is of interest for the wine industry for technological and sensory reasons. However, the interactions between these yeasts are not well understood, especially those regarding the availability of nutrients. The aim of the present study was to analyze the effect of nitrogen and sugar concentration on the evolution of mixed yeast populations on controlled laboratory-scale fermentations monitored by density, plate culturing, PCR-DGGE and sugar and nitrogen consumption. Furthermore, the effect of the time of inoculation of Saccharomyces cerevisiae respect the initial co-inoculation of three non-Saccharomyces yeasts was evaluated over the evolution of fermentation. Our results have shown that S. cerevisiae inoculation during the first 48 h conferred a stabilizing effect over the fermentations with non-Saccharomyces strains tested and, generally, reduced yeast diversity at the end of the fermentation. On the other hand, nitrogen limitation increased the time of fermentation and also the proportion of non-Saccharomyces yeasts at mid and final fermentation. High sugar concentration resulted in different proportions of the inoculated yeast depending on the time of S. cerevisiae inoculation. This work emphasizes the importance of the concentration of nutrients on the evolution of mixed fermentations and points to the optimal conditions for a stable fermentation in which the inoculated yeasts survived until the end.
  • Others:

    Author, as appears in the article.: Albert Mas; Jessica Lleixà; Maria Manzano; María del C. Portillo
    Department: Bioquímica i Biotecnologia
    URV's Author/s: MAS BARON, ALBERTO; Jessica Lleixà; Maria Manzano; PORTILLO GUISADO, MARIA DEL CARMEN
    Keywords: Torulaspora Wine Hanseniaspora Fermentation Starmarella
    Abstract: The inoculation of wines with autochthonous yeast allows obtaining complex wines with a peculiar microbial footprint characteristic from a wine region. Mixed inoculation of non-Saccharomyces yeasts and S. cerevisiae is of interest for the wine industry for technological and sensory reasons. However, the interactions between these yeasts are not well understood, especially those regarding the availability of nutrients. The aim of the present study was to analyze the effect of nitrogen and sugar concentration on the evolution of mixed yeast populations on controlled laboratory-scale fermentations monitored by density, plate culturing, PCR-DGGE and sugar and nitrogen consumption. Furthermore, the effect of the time of inoculation of Saccharomyces cerevisiae respect the initial co-inoculation of three non-Saccharomyces yeasts was evaluated over the evolution of fermentation. Our results have shown that S. cerevisiae inoculation during the first 48 h conferred a stabilizing effect over the fermentations with non-Saccharomyces strains tested and, generally, reduced yeast diversity at the end of the fermentation. On the other hand, nitrogen limitation increased the time of fermentation and also the proportion of non-Saccharomyces yeasts at mid and final fermentation. High sugar concentration resulted in different proportions of the inoculated yeast depending on the time of S. cerevisiae inoculation. This work emphasizes the importance of the concentration of nutrients on the evolution of mixed fermentations and points to the optimal conditions for a stable fermentation in which the inoculated yeasts survived until the end.
    Research group: Biotecnologia Enològica
    Thematic Areas: Enologia Enología Oenology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-302X
    Author identifier: 0000-0002-0763-1679; n/a; n/a; 0000-0002-4576-0244
    Record's date: 2017-01-24
    Journal volume: 7
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2016.01959/full
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: https://doi.org/10.3389/fmicb.2016.01959
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    First page: Art.num. 1959
    Publication Type: Article Artículo Article
  • Keywords:

    Vi -- Fermentació
    Starmarella
    Torulaspora
    Wine
    Hanseniaspora
    Fermentation
    Starmarella
    Enologia
    Enología
    Oenology
    1664-302X
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