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DETERMINATION OF TRICHOTHECENES IN CEREAL MATRICES USING SUBCRITICAL WATER EXTRACTION FOLLOWED BY SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

  • Identification data

    Identifier: PC:3409
    Authors:
    Fontanals, N.Miró, E.Herrero, P.Canela, N.Arola, Ll.Ras, R.Borrull, F.
    Abstract:
    Subcritical water extraction followed by solid-phase extraction and ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection is reported for the first time for the determination of 6 trichothecenes (deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, HT-2 toxin and T-2 toxin) from different cereals. Water with 1% formic acid was used as the extraction solvent followed by a solid-phase extraction clean-up, achieving good performance with acceptable extraction recoveries, method detection limits between 0.05 μg kg-1 and 4.0 μg kg-1, and method quantification limits between 0.4 μg kg-1 and 20 μg kg-1. The use of water as the extraction solvent allowed a selective extraction affording low matrix effect levels, and the detection and quantification of natural target trichothecenes at very low concentration levels. This extraction method was applied to different cereals, a pseudocereal and an oilseed sample, of which maize, millet and oat were contaminated by at least one trichothecene.
  • Others:

    Author, as appears in the article.: Fontanals, N.; Miró, E.; Herrero, P.; Canela, N.; Arola, Ll.; Ras, R.; Borrull, F.
    Department: Química Analítica i Química Orgànica
    URV's Author/s: FONTANALS TORROJA, NÚRIA; Miró, E.; Herrero, P.; Canela, N.; Arola, Ll.; Ras, R.; Borrull, F.
    Abstract: Subcritical water extraction followed by solid-phase extraction and ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection is reported for the first time for the determination of 6 trichothecenes (deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, HT-2 toxin and T-2 toxin) from different cereals. Water with 1% formic acid was used as the extraction solvent followed by a solid-phase extraction clean-up, achieving good performance with acceptable extraction recoveries, method detection limits between 0.05 μg kg-1 and 4.0 μg kg-1, and method quantification limits between 0.4 μg kg-1 and 20 μg kg-1. The use of water as the extraction solvent allowed a selective extraction affording low matrix effect levels, and the detection and quantification of natural target trichothecenes at very low concentration levels. This extraction method was applied to different cereals, a pseudocereal and an oilseed sample, of which maize, millet and oat were contaminated by at least one trichothecene.
    Research group: Grup de Cromatografia. Aplicacions Mediambientals
    Thematic Areas: Química Química Chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1936-9751
    Author identifier: 0000-0002-7534-1964; ; ; ; ; ;
    Record's date: 2019-01-30
    Last page: 1121
    Journal volume: 11
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://link.springer.com/article/10.1007/s12161-017-1089-6
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1007/s12161-017-1089-6
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    First page: 1113
    Publication Type: Article Artículo Article
  • Keywords:

    Micotoxines
    Química
    Química
    Chemistry
    1936-9751
  • Documents:

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