DETERMINATION OF TRICHOTHECENES IN CEREAL MATRICES USING SUBCRITICAL WATER EXTRACTION FOLLOWED BY SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

Identifier:  PC:3409

Handle: https://hdl.handle.net/20.500.11797/PC3409

Authors:  Fontanals, N.; Miró, E.; Herrero, P.; Canela, N.; Arola, Ll.; Ras, R.; Borrull, F.

Abstract:
Subcritical water extraction followed by solid-phase extraction and ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection is reported for the first time for the determination of 6 trichothecenes (deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, HT-2 toxin and T-2 toxin) from different cereals. Water with 1% formic acid was used as the extraction solvent followed by a solid-phase extraction clean-up, achieving good performance with acceptable extraction recoveries, method detection limits between 0.05 μg kg-1 and 4.0 μg kg-1, and method quantification limits between 0.4 μg kg-1 and 20 μg kg-1. The use of water as the extraction solvent allowed a selective extraction affording low matrix effect levels, and the detection and quantification of natural target trichothecenes at very low concentration levels. This extraction method was applied to different cereals, a pseudocereal and an oilseed sample, of which maize, millet and oat were contaminated by at least one trichothecene.