e-ISSN: 1422-0067
Keywords: Simple steatosis Non-alcoholic steatohepatitis Non-alcoholic fatty liver disease Morbid obesity Insulin resistance Fatty acid metabolism
Research group: Grup d'Estudi de Malalties Metabòliques Associades a Insulin Resistència (GEMMAIR)
Record's date: 2024-09-28
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: International Journal Of Molecular Sciences. 15 (12): 22173-22187
APA: Auguet, Teresa; Berlanga, Alba; Guiu-Jurado, Esther; Martinez, Salome; Porras, Jose Antonio; Aragones, Gemma; Sabench, Fatima; Hernandez, Merce; Aguil (2014). Altered fatty acid metabolism-related gene expression in liver from morbidly obese women with non-alcoholic fatty liver disease. International Journal Of Molecular Sciences, 15(12), 22173-22187. DOI: 10.3390/ijms151222173
First page: 22173
Publication Type: Journal Publications
Author, as appears in the article.: Auguet, Teresa; Berlanga, Alba; Guiu-Jurado, Esther; Martinez, Salome; Porras, Jose Antonio; Aragones, Gemma; Sabench, Fatima; Hernandez, Merce; Aguilar, Carmen; Sirvent, Joan Josep; Del Castillo, Daniel; Richart, Cristobal
Department: Medicina i Cirurgia
URV's Author/s: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima / SIRVENT CA
Abstract: Lipid accumulation in the human liver seems to be a crucial mechanism in the pathogenesis and the progression of non-alcoholic fatty liver disease (NAFLD). We aimed to evaluate gene expression of different fatty acid (FA) metabolism-related genes in morbidly obese (MO) women with NAFLD. Liver expression of key genes related to de novo FA synthesis (LXRα, SREBP1c, ACC1, FAS), FA uptake and transport (PPARγ, CD36, FABP4), FA oxidation (PPARα), and inflammation (IL6, TNFα, CRP, PPARδ) were assessed by RT-qPCR in 127 MO women with normal liver histology (NL, n = 13), simple steatosis (SS, n = 47) and non-alcoholic steatohepatitis (NASH, n = 67). Liver FAS mRNA expression was significantly higher in MO NAFLD women with both SS and NASH compared to those with NL (p = 0.003, p = 0.010, respectively). Hepatic IL6 and TNFα mRNA expression was higher in NASH than in SS subjects (p = 0.033, p = 0.050, respectively). Interestingly, LXRα, ACC1 and FAS expression had an inverse relation with the grade of steatosis. These results were confirmed by western blot analysis. In conclusion, our results indicate that lipogenesis seems to be downregulated in advanced stages of SS, suggesting that, in this type of extreme obesity, the deregulation of the lipogenic pathway might be associated with the severity of steatosis.
Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
licence for use: thttps://creativecommons.org/licenses/by/3.0/es/
ISSN: 14220067
Author's mail: joseantonio.porras@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
Last page: 22187
Journal volume: 15
Link to the original source: https://www.mdpi.com/1422-0067/15/12/22173
Article's DOI: 10.3390/ijms151222173
Entity: Universitat Rovira i Virgili
Journal publication year: 2014