Articles producció científica> Medicina i Cirurgia

miR33a/miR33b* and miR122 as Possible Contributors to Hepatic Lipid Metabolism in Obese Women with nonalcoholic Fatty Liver Disease

  • Identification data

    Identifier: imarina:3654303
    Authors:
    Auguet, TeresaAragones, GemmaBerlanga, AlbaGuiu-Jurado, EstherMarti, AndreuMartinez, SalomeSabench, FatimaHernandez, MerceAguilar, CarmenSirvent, Joan Josepdel Castillo, DanielRichart, Cristobal
    Abstract:
    Specific miRNA expression profiles have been shown to be associated with nonalcoholic fatty liver disease (NAFLD). We examined the correlation between the circulating levels and hepatic expression of miR122 and miR33a/b*, the key lipid metabolism-related gene expression and the clinicopathological factors of obese women with NAFLD. We measured miR122 and miR33a/b* expression in liver samples from 62 morbidly obese (MO), 30 moderately obese (ModO), and eight normal-weight controls. MiR122 and miR33a/b* expression was analyzed by qRT-PCR. Additionally, miR122 and miR33b* circulating levels were analyzed in 122 women. Hepatic miR33b* expression was increased in MO compared to ModO and controls, whereas miR122 expression was decreased in the MO group compared to ModO. In obese cohorts, miR33b* expression was increased in nonalcoholic steatohepatitis (NASH). Regarding circulating levels, MO patients with NASH showed higher miR122 levels than MO with simple steatosis (SS). These circulating levels are good predictors of histological features associated with disease severity. MO is associated with altered hepatic miRNA expression. In obese women, higher miR33b* liver expression is associated with NASH. Moreover, multiple correlations between miRNAs and the expression of genes related to lipid metabolism were found, that would suggest a miRNA-host gene circuit. Finally, miR122 circulating levels could be included in a panel of different biomarkers to improve accuracy in the non-invasive diagnosis of NASH.
  • Others:

    Author, as appears in the article.: Auguet, Teresa; Aragones, Gemma; Berlanga, Alba; Guiu-Jurado, Esther; Marti, Andreu; Martinez, Salome; Sabench, Fatima; Hernandez, Merce; Aguilar, Carmen; Sirvent, Joan Josep; del Castillo, Daniel; Richart, Cristobal
    Department: Medicina i Cirurgia Bioquímica i Biotecnologia Ciències Mèdiques Bàsiques
    e-ISSN: 1422-0067
    URV's Author/s: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima / SIRVENT CALVERA, JUAN JOSÉ
    Keywords: Obesity Nonalcoholic fatty liver disease Mir33a/b* Mir122 nonalcoholic fatty liver disease mir33a/b* mir122
    Abstract: Specific miRNA expression profiles have been shown to be associated with nonalcoholic fatty liver disease (NAFLD). We examined the correlation between the circulating levels and hepatic expression of miR122 and miR33a/b*, the key lipid metabolism-related gene expression and the clinicopathological factors of obese women with NAFLD. We measured miR122 and miR33a/b* expression in liver samples from 62 morbidly obese (MO), 30 moderately obese (ModO), and eight normal-weight controls. MiR122 and miR33a/b* expression was analyzed by qRT-PCR. Additionally, miR122 and miR33b* circulating levels were analyzed in 122 women. Hepatic miR33b* expression was increased in MO compared to ModO and controls, whereas miR122 expression was decreased in the MO group compared to ModO. In obese cohorts, miR33b* expression was increased in nonalcoholic steatohepatitis (NASH). Regarding circulating levels, MO patients with NASH showed higher miR122 levels than MO with simple steatosis (SS). These circulating levels are good predictors of histological features associated with disease severity. MO is associated with altered hepatic miRNA expression. In obese women, higher miR33b* liver expression is associated with NASH. Moreover, multiple correlations between miRNAs and the expression of genes related to lipid metabolism were found, that would suggest a miRNA-host gene circuit. Finally, miR122 circulating levels could be included in a panel of different biomarkers to improve accuracy in the non-invasive diagnosis of NASH.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14220067
    Author's mail: mariasalome.martinez@urv.cat danieldel.castillo@urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Journal volume: 17
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/17/10/1620
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 17 (10): 1620-
    APA: Auguet, Teresa; Aragones, Gemma; Berlanga, Alba; Guiu-Jurado, Esther; Marti, Andreu; Martinez, Salome; Sabench, Fatima; Hernandez, Merce; Aguilar, Car (2016). miR33a/miR33b* and miR122 as Possible Contributors to Hepatic Lipid Metabolism in Obese Women with nonalcoholic Fatty Liver Disease. International Journal Of Molecular Sciences, 17(10), 1620-. DOI: 10.3390/ijms17101620
    Article's DOI: 10.3390/ijms17101620
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Obesity
    Nonalcoholic fatty liver disease
    Mir33a/b*
    Mir122
    nonalcoholic fatty liver disease
    mir33a/b*
    mir122
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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