Author, as appears in the article.: Parra, Sandra; Marsillach, Judit; Aragones, Gerard; Rull, Anna; Beltran-Debon, Raul; Alonso-Villaverde, Carlos; Joven, Jorge; Camps, Jordi;
Department: Medicina i Cirurgia
URV's Author/s: Aragonès Bargalló, Gerard / Beltrán Debón, Raúl Alejandro / Camps Andreu, Jorge / Joven Maried, Jorge / MARSILLACH LÓPEZ, JUDIT / Parra Pérez, Sandra / RULL AIXA, ANNA
Keywords: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu
Abstract: Background. Paraoxonase-1 (PON1) is an antioxidant enzyme that attenuates the production of the monocyte chemoattractant protein-1 (MCP-1) in vitro. Although oxidation and inflammation are closely related processes, the association between PON1 and MCP-1 has not been completely characterised due, probably, to that the current use of synthetic substrates for PON1 measurement limits the interpretation of the data. In the present study, we explored the relationships between the circulating levels of PON1 and MCP-1 in human immunodeficiency virus-infected patients in relation to the multifunctional capabilities of PON1. Methods. We measured selected variables in 227 patients and in a control group of 409 participants. Serum PON1 esterase and lactonase activities were measured as the rates of hydrolysis of paraoxon and of 5-(thiobutyl)-butyrolactone, respectively. Oxidised LDL and MCP-1 concentrations were determined by enzyme-linked immunosorbent assay. High-density lipoproteins cholesterol, apolipoprotein A-I, and C-reactive protein concentrations were measured by standard automated methods. Results. There were significant relationships between PON1 activity and several indices of oxidation and inflammation in control subjects and in infected patients. However, these relationships varied not only with disease status but also on the type of substrate used for PON1 measurement. Conclusion. The present study is a cautionary tale highlighting that results of clinical studies on PON1 may vary depending on the methods used as well as the disease studied. Until more specific methods using physiologically-akin substrates are developed for PON1 measurement, we suggest the simultaneous employment of at least two different substrates in order to improve the reliability of the results obtained. © 2010 Parra et al; licensee BioMed Central Ltd.
Thematic Areas: Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Enfermagem Endocrinology, diabetes and metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1476-511X
Author's mail: jorge.camps@urv.cat sandra.parra@urv.cat jorge.joven@urv.cat gerard.aragones@urv.cat raul.beltran@urv.cat
Author identifier: 0000-0002-3165-3640 0000-0001-9363-6574 0000-0003-2749-4541 0000-0001-9691-1906
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Lipids In Health And Disease. 9 (32): 32-
APA: Parra, Sandra; Marsillach, Judit; Aragones, Gerard; Rull, Anna; Beltran-Debon, Raul; Alonso-Villaverde, Carlos; Joven, Jorge; Camps, Jordi; (2010). Methodological constraints in interpreting serum paraoxonase-1 activity measurements: An example from a study in HIV-infected patients. Lipids In Health And Disease, 9(32), 32-. DOI: 10.1186/1476-511X-9-32
Entity: Universitat Rovira i Virgili
Journal publication year: 2010
Publication Type: Journal Publications
Repositori URV