Author, as appears in the article.: Ceperuelo-Mallafre, Victoria; Ejarque, Miriam; Duran, Xavier; Pachon, Gisela; Vazquez-Carballo, Ana; Roche, Kelly; Nunez-Roa, Catalina; Garrido-Sanchez, Lourdes; Tinahones, Francisco J.; Vendrell, Joan; Fernandez-Veledo, Sonia;
Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia
URV's Author/s: Ceperuelo Mallafré, Maria Victoria / Fernandez Veledo, Sonia / Vendrell Ortega, Juan José
Keywords: Zinc-alpha(2)-glycoprotein Weight Skeletal-muscle Resistance Pathways Necrosis-factor-alpha Lipolysis Lipid mobilizing factor Glucose-uptake Adipose-tissue
Abstract: Objective: Evidence from mouse models suggests that zinc-α2-glycoprotein (ZAG) is a novel anti-obesity adipokine. In humans, however, data are controversial and its physiological role in adipose tissue (AT) remains unknown. Here we explored the molecular mechanisms by which ZAG regulates carbohydrate metabolism in human adipocytes. Methods: ZAG action on glucose uptake and insulin action was analyzed. β1 and β2-adrenoreceptor (AR) antagonists and siRNA targeting PP2A phosphatase were used to examine the mechanisms by which ZAG modulates insulin sensitivity. Plasma levels of ZAG were measured in a lean patient cohort stratified for HOMA-IR. Results: ZAG treatment increased basal glucose uptake, correlating with an increase in GLUT expression, but induced insulin resistance in adipocytes. Pretreatment of adipocytes with propranolol and a specific β1-AR antagonist demonstrated that ZAG effects on basal glucose uptake and GLUT4 expression are mediated via β1-AR, whereas inhibition of insulin action is dependent on β2-AR activation. ZAG treatment correlated with an increase in PP2A activity. Silencing of the PP2A catalytic subunit abrogated the negative effect of ZAG on insulinstimulated AKT phosphorylation and glucose uptake but not on GLUT4 expression and basal glucose uptake. ZAG circulating levels were unchanged in a lean patient cohort stratified for HOMA-IR. Neither glucose nor insulin was associated with plasma ZAG. Conclusions: ZAG inhibits insulin-induced glucose uptake in human adipocytes by impairing insulin signaling at the level of AKT in a β2-AR- and PP2A-dependent manner. © 2015 Ceperuelo-Mallafré et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: victoria.ceperuelo@urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
Author identifier: 0000-0002-4460-9761 0000-0003-2906-3788 0000-0002-6994-6115
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129644
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Plos One. 10 (6): e0129644-
APA: Ceperuelo-Mallafre, Victoria; Ejarque, Miriam; Duran, Xavier; Pachon, Gisela; Vazquez-Carballo, Ana; Roche, Kelly; Nunez-Roa, Catalina; Garrido-Sanche (2015). Zinc-alpha 2-Glycoprotein Modulates AKT-Dependent Insulin Signaling in Human Adipocytes by Activation of the PP2A Phosphatase. Plos One, 10(6), e0129644-. DOI: 10.1371/journal.pone.0129644
Article's DOI: 10.1371/journal.pone.0129644
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications
Repositori URV