Author, as appears in the article.: Karri V; Kumar V; Ramos D; Oliveira E; Schuhmacher M
Department: Enginyeria Química
URV's Author/s: Kumar, Vikas / Schuhmacher Ansuategui, Marta
Keywords: Ht-22 cell line Heavy metals in vitro toxicity Dose response Dna damage Apoptosis
Abstract: © 2017, Springer Science+Business Media, LLC. Heavy metals are considered some of the most toxic environmental pollutants. Exposure to heavy metals including lead (Pb), cadmium (Cd), arsenic (As), and methyl mercury (MeHg) has long been known to cause damage to human health. Many recent studies have supported the hippocampus as the major target for these four metals for inflicting cognitive dysfunction. In the present study, we proposed hippocampal relevant in vitro toxicity of Pb, Cd, As, and MeHg in HT-22 cell line. This study reports, initially, cytotoxic effects in acute, subchronic, chronic exposures. We further investigated the mechanistic potency of DNA damage and apoptosis damage with the observed cytotoxicity. The genotoxicity and apoptosis were measured by using the comet assay, annexin-V FTIC / propidium iodide (PI) assay, respectively. The results of cytotoxicity assay clearly demonstrated significant concentration and time-dependent effects on HT-22 cell line. The genotoxic and apoptosis effects also concentration-dependent fashion with respect to their potency in the range of IC 10 –IC 30, maximal level of damage observed in MeHg. In conclusion, the obtained result suggests concentration and potency-dependent response; the maximal level of toxicity was observed in MeHg. These novel findings support that Pb, Cd, As, and MeHg induce cytotoxic, genotoxic, and apoptotic effects on HT-22 cells in potency-dependent manner; MeHg> As> Cd> Pb. Therefore, the toxicity of Pb, Cd, As, and MeHg could be useful for knowing the common underlying molecular mechanism, and also for estimating the mixture impacts on HT-22 cell line.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry General medicine Farmacia Engenharias ii Engenharias i Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology Biochemistry Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vikas.kumar@urv.cat marta.schuhmacher@urv.cat
Author identifier: 0000-0002-9795-5967 0000-0003-4381-2490
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://link.springer.com/article/10.1007/s12011-017-1177-x
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biological Trace Element Research. 184 (1): 226-239
APA: Karri V; Kumar V; Ramos D; Oliveira E; Schuhmacher M (2018). Comparative In Vitro Toxicity Evaluation of Heavy Metals (Lead, Cadmium, Arsenic, and Methylmercury) on HT-22 Hippocampal Cell Line. Biological Trace Element Research, 184(1), 226-239. DOI: 10.1007/s12011-017-1177-x
Article's DOI: 10.1007/s12011-017-1177-x
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications