Effect of radiotherapy on the expression of cardiovascular disease-related miRNA-146a, -155, -221 and -222 in blood of women with breast cancer

Author, as appears in the article.: Esplugas, Roser; Arenas, Meritxell; Serra, Noemi; Belles, Montserrat; Bonet, Marta; Gascon, Marina; Vallve, Joan-Cartes; Linares, Victoria

Department: Ciències Mèdiques Bàsiques; Medicina i Cirurgia

URV's Author/s: Arenas Prat, Meritxell / Bellés Mateu, Montserrat / Esplugas Borràs, Roser / Linares Vidal, M. Victoria / Serra Encinas, Noemi / Vallvé Torrente, Joan Carles

Keywords: Suppression; Rnas; Risk; Radiotherapy, adjuvant; Radiotherapy; Radiation injuries; Potential biomarker; Organs at risk; Mirnas plasmáticos; Mirn222 microrna, human; Mirn221 microrna, human; Mirn155 microrna, human; Mirn146 microrna, human; Mir-221/222; Mir-146a; Middle aged; Micrornas; Mastectomy; Humans; Heart; Gene expression profiling; Female; Dose fractionation, radiation; Disease progression; Coronary-heart-disease; Circulating micrornas; Chemoradiotherapy, adjuvant; Cardiovascular diseases; Breast neoplasms; Breast cancer; Biomarkers; Artery; Altered expression; Aged, 80 and over; Aged; Adult

Abstract: © 2019 Esplugas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Breast cancer (BC) is one of the most important neoplasias among women. Many patients receive radiotherapy (RT), which involves radiation exposure of the thoracic zone, including the heart and blood vessels, leading to the development of cardiovascular disease (CVD) as a long-term side effect. The severity of CVD-related pathologies leads research on assessing novel CVD biomarkers as diagnostic, prognostic or therapeutic agents. Currently, the possible candidates include blood microRNAs (miRNAs). Previous studies have supported a role for miRNA-146a, -155, -221, and -222 in the progression of CVD. Our purpose was to evaluate the RT-induced modulation of the expression of these miRNAs in the blood of women with BC. Pre-RT control and post-RT blood samples were collected, and after miRNA isolation and reverse transcription, the levels of the selected miRNAs were measured by real-time PCR. Our results showed that miRNA-155 exhibited the lowest expression, while miRNA-222 exhibited the highest expression, followed by miRNA-221. The expression of each individual miRNA was positively correlated with that of the others both pre-RT control and post-RT and inversely correlated with age before RT. Furthermore, RT promoted the overexpression of the selected miRNAs. Their levels were also affected by CVD-linked clinical parameters, treatment and BC side. Modulation of the expression of the selected miRNAs together with other risk factors might be associated with the development of future cardiovascular pathologies. Further confirmatory studies are needed to assess their potential as possible biomarkers in the progression of or as therapeutic targets for RT-induced CVD in BC patients.

Thematic Areas: Zootecnia / recursos pesqueiros; Sociology; Sociología; Serviço social; Saúde coletiva; Química; Psychology; Psicología; Planejamento urbano e regional / demografia; Odontología; Nutrição; Multidisciplinary sciences; Multidisciplinary; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Linguística e literatura; Letras / linguística; Interdisciplinary research in the social sciences; Interdisciplinar; Human geography and urban studies; History & philosophy of science; Historia; Geografía; Geociências; General medicine; General biochemistry,genetics and molecular biology; General agricultural and biological sciences; Farmacia; Environmental studies; Ensino; Engenharias iv; Engenharias iii; Engenharias ii; Engenharias i; Enfermagem; Educação física; Educação; Economia; Direito; Demography; Comunicação e informação; Ciências sociais aplicadas i; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência política e relações internacionais; Ciência de alimentos; Ciência da computação; Biotecnología; Biology; Biodiversidade; Biochemistry, genetics and molecular biology (miscellaneous); Astronomia / física; Arquitetura, urbanismo e design; Archaeology; Antropologia / arqueologia; Anthropology; Agricultural and biological sciences (miscellaneous); Administração, ciências contábeis e turismo; Administração pública e de empresas, ciências contábeis e turismo

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 19326203

Author's mail: roser.esplugas@urv.cat; jc.vallve@urv.cat; meritxell.arenas@urv.cat; noemi.serra@urv.cat; mvictoria.linares@urv.cat; jc.vallve@urv.cat; montserrat.belles@urv.cat

Record's date: 2025-03-15

Paper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0217443

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Paper original source: Plos One. 14 (5): e0217443-

APA: Esplugas, Roser; Arenas, Meritxell; Serra, Noemi; Belles, Montserrat; Bonet, Marta; Gascon, Marina; Vallve, Joan-Cartes; Linares, Victoria (2019). Effect of radiotherapy on the expression of cardiovascular disease-related miRNA-146a, -155, -221 and -222 in blood of women with breast cancer. Plos One, 14(5), e0217443-. DOI: 10.1371/journal.pone.0217443

Article's DOI: 10.1371/journal.pone.0217443

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications