Articles producció científica> Ciències Mèdiques Bàsiques

International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for Fusarium Species Identified by Molecular Methods for the CLSI Broth Microdilution Method

  • Identification data

    Identifier: imarina:6063288
    Authors:
    Espinel-Ingroff, A.Colombo, A. L.Cordoba, S.Dufresne, P. J.Fuller, J.Ghannoum, M.Gonzalez, G. M.Guarro, J.Kidd, S. E.Meis, J. F.Melhem, T. M. S. C.Pelaez, T.Pfaller, M. A.Szeszs, M. W.Takahaschi, J. P.Tortorano, A. M.Wiederhold, N. P.Turnidger, J.
    Abstract:
    The CLSI epidemiological cutoff values (ECVs) of antifungal agents are available for various Candida spp., Aspergillus spp., and the Mucorales. However, those categorical endpoints have not been established for Fusarium spp., mostly due to the difficulties associated with collecting sufficient CLSI MICs for clinical isolates identified according to the currently recommended molecular DNA-PCR-based identification methodologies. CLSI MIC distributions were established for 53 Fusarium dimerum species complex (SC), 10 F. fujikuroi, 82 F. proliferatum, 20 F. incarnatum-F. equiseti SC, 226 F. oxysporum SC, 608 F. solani SC, and 151 F. verticillioides isolates originating in 17 laboratories (in Argentina, Australia, Brazil, Canada, Europe, Mexico, and the United States). According to the CLSI guidelines for ECV setting, ECVs encompassing >= 97.5% of pooled statistically modeled MIC distributions were as follows: for amphotericin B, 4 mu g/ml (F. verticillioides) and 8 mu g/ml (F. oxysporum SC and F. solani SC); for posaconazole, 2 mu g/ml (F. verticillioides), 8 mu g/ml (F. oxysporum SC), and 32 mu g/ml (F. solani SC); for voriconazole, 4 mu g/ml (F. verticillioides), 16 mu g/ml (F. oxysporum SC), and 32 mu g/ml (F. solani SC); and for itraconazole, 32 mu g/ml (F. oxysporum SC and F. solani SC). Insufficient data precluded ECV definition for the other species. Although these ECVs could aid in detecting non-wild-type isolates with reduced susceptibility to the agents evaluated, the relationship between molecular mechanisms of resistance (gene mutations) and MICs still needs to be investigated for Fusarium spp.
  • Others:

    Author, as appears in the article.: Espinel-Ingroff, A.; Colombo, A. L.; Cordoba, S.; Dufresne, P. J.; Fuller, J.; Ghannoum, M.; Gonzalez, G. M.; Guarro, J.; Kidd, S. E.; Meis, J. F.; Melhem, T. M. S. C.; Pelaez, T.; Pfaller, M. A.; Szeszs, M. W.; Takahaschi, J. P.; Tortorano, A. M.; Wiederhold, N. P.; Turnidger, J.;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Guarro Artigas, Josep
    Keywords: Voriconazole Spp. Resistance Posaconazole Multicenter Itraconazole Invasive fusariosis Infections In-vitro Amphotericin-b
    Abstract: The CLSI epidemiological cutoff values (ECVs) of antifungal agents are available for various Candida spp., Aspergillus spp., and the Mucorales. However, those categorical endpoints have not been established for Fusarium spp., mostly due to the difficulties associated with collecting sufficient CLSI MICs for clinical isolates identified according to the currently recommended molecular DNA-PCR-based identification methodologies. CLSI MIC distributions were established for 53 Fusarium dimerum species complex (SC), 10 F. fujikuroi, 82 F. proliferatum, 20 F. incarnatum-F. equiseti SC, 226 F. oxysporum SC, 608 F. solani SC, and 151 F. verticillioides isolates originating in 17 laboratories (in Argentina, Australia, Brazil, Canada, Europe, Mexico, and the United States). According to the CLSI guidelines for ECV setting, ECVs encompassing >= 97.5% of pooled statistically modeled MIC distributions were as follows: for amphotericin B, 4 mu g/ml (F. verticillioides) and 8 mu g/ml (F. oxysporum SC and F. solani SC); for posaconazole, 2 mu g/ml (F. verticillioides), 8 mu g/ml (F. oxysporum SC), and 32 mu g/ml (F. solani SC); for voriconazole, 4 mu g/ml (F. verticillioides), 16 mu g/ml (F. oxysporum SC), and 32 mu g/ml (F. solani SC); and for itraconazole, 32 mu g/ml (F. oxysporum SC and F. solani SC). Insufficient data precluded ECV definition for the other species. Although these ECVs could aid in detecting non-wild-type isolates with reduced susceptibility to the agents evaluated, the relationship between molecular mechanisms of resistance (gene mutations) and MICs still needs to be investigated for Fusarium spp.
    Thematic Areas: Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Infectious diseases Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00664804
    Author's mail: josep.guarro@urv.cat
    Author identifier: 0000-0002-7839-7568
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://journals.asm.org/doi/10.1128/aac.02456-15
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Antimicrobial Agents And Chemotherapy. 60 (2): 1079-1084
    APA: Espinel-Ingroff, A.; Colombo, A. L.; Cordoba, S.; Dufresne, P. J.; Fuller, J.; Ghannoum, M.; Gonzalez, G. M.; Guarro, J.; Kidd, S. E.; Meis, J. F.; Me (2016). International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for Fusarium Species Identified by Molecular Methods for the CLSI Broth Microdilution Method. Antimicrobial Agents And Chemotherapy, 60(2), 1079-1084. DOI: 10.1128/AAC.02456-15
    Article's DOI: 10.1128/AAC.02456-15
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Infectious Diseases,Microbiology,Pharmacology,Pharmacology & Pharmacy,Pharmacology (Medical)
    Voriconazole
    Spp.
    Resistance
    Posaconazole
    Multicenter
    Itraconazole
    Invasive fusariosis
    Infections
    In-vitro
    Amphotericin-b
    Saúde coletiva
    Química
    Pharmacology (medical)
    Pharmacology & pharmacy
    Pharmacology
    Odontología
    Nutrição
    Microbiology
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Infectious diseases
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Educação física
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Astronomia / física
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