Articles producció científica> Medicina i Cirurgia

Endocannabinoid receptors gene expression in morbidly obese women with nonalcoholic fatty liver disease

  • Identification data

    Identifier: imarina:6387793
    Authors:
    Auguet, TeresaBerlanga, AlbaGuiu-Jurado, EstherTerra, XimenaMartinez, SalomeAguilar, CarmenFiliu, ElisaAlibalic, AjlaSabench, FatimaHernandez, MerceDel Castillo, DanielRichart, Cristbal
    Abstract:
    Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n=16), simple steatosis (SS, n=28), and nonalcoholic steatohepatitis (NASH, n=28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study. © 2014 Teresa Auguet et al.
  • Others:

    Author, as appears in the article.: Auguet, Teresa; Berlanga, Alba; Guiu-Jurado, Esther; Terra, Ximena; Martinez, Salome; Aguilar, Carmen; Filiu, Elisa; Alibalic, Ajla; Sabench, Fatima; Hernandez, Merce; Del Castillo, Daniel; Richart, Cristbal
    Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia Bioquímica i Biotecnologia
    URV's Author/s: Aguilar Crespillo, Carmen Isabel / Alibalic, Ajla / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima / Terra Barbadora, Ximena
    Abstract: Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n=16), simple steatosis (SS, n=28), and nonalcoholic steatohepatitis (NASH, n=28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study. © 2014 Teresa Auguet et al.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Geografía General immunology and microbiology General biochemistry,genetics and molecular biology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biotechnology & applied microbiology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física Antropologia / arqueologia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 23146133
    Author's mail: mariasalome.martinez@urv.cat danieldel.castillo@urv.cat ajla.alibalic@estudiants.urv.cat ximena.terra@urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-1043-5844 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.hindawi.com/journals/bmri/2014/502542/
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Biomed Research International. 2014 502542-
    APA: Auguet, Teresa; Berlanga, Alba; Guiu-Jurado, Esther; Terra, Ximena; Martinez, Salome; Aguilar, Carmen; Filiu, Elisa; Alibalic, Ajla; Sabench, Fatima; (2014). Endocannabinoid receptors gene expression in morbidly obese women with nonalcoholic fatty liver disease. Biomed Research International, 2014(), 502542-. DOI: 10.1155/2014/502542
    Article's DOI: 10.1155/2014/502542
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2014
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Biotechnology & Applied Microbiology,Immunology and Microbiology (Miscellaneous),Medicine (Miscellaneous),Medicine, Research & Experimental
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Odontología
    Nutrição
    Medicine, research & experimental
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Geografía
    General immunology and microbiology
    General biochemistry,genetics and molecular biology
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Enfermagem
    Educação física
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biotechnology & applied microbiology
    Biodiversidade
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
    Astronomia / física
    Antropologia / arqueologia
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