Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Nucleotide depletion reveals the impaired ribosome biogenesis checkpoint as a barrier against DNA damage

  • Identification data

    Identifier: imarina:6390432
    Handle: http://hdl.handle.net/20.500.11797/imarina6390432
  • Authors:

    Pelletier J., Riaño-Canalias F., Almacellas E., Mauvezin C., Samino S., Feu S., Menoyo S., Domostegui A., Garcia-Cajide M., Salazar R., Cortés C., Marcos R., Tauler A., Yanes O., Agell N., Kozma S.C., Gentilella A., Thomas G.
  • Others:

    Author, as appears in the article.: Pelletier J., Riaño-Canalias F., Almacellas E., Mauvezin C., Samino S., Feu S., Menoyo S., Domostegui A., Garcia-Cajide M., Salazar R., Cortés C., Marcos R., Tauler A., Yanes O., Agell N., Kozma S.C., Gentilella A., Thomas G.
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: SAMINO GENÉ, SARA / Yanes Torrado, Óscar
    Keywords: Suppression S-phase Rna-synthesis Proliferation P53 P21 Nucleotides Myc Metabolism Irbc Impdh Dna-damage Cell-cycle arrest Cancer Activation p21 nucleotides impdh irbc
    Abstract: ©2020 The Authors. Published under the terms of the CC BY 4.0 license Many oncogenes enhance nucleotide usage to increase ribosome content, DNA replication, and cell proliferation, but in parallel trigger p53 activation. Both the impaired ribosome biogenesis checkpoint (IRBC) and the DNA damage response (DDR) have been implicated in p53 activation following nucleotide depletion. However, it is difficult to reconcile the two checkpoints operating together, as the IRBC induces p21-mediated G1 arrest, whereas the DDR requires that cells enter S phase. Gradual inhibition of inosine monophosphate dehydrogenase (IMPDH), an enzyme required for de novo GMP synthesis, reveals a hierarchical organization of these two checkpoints. We find that the IRBC is the primary nucleotide sensor, but increased IMPDH inhibition leads to p21 degradation, compromising IRBC-mediated G1 arrest and allowing S phase entry and DDR activation. Disruption of the IRBC alone is sufficient to elicit the DDR, which is strongly enhanced by IMPDH inhibition, suggesting that the IRBC acts as a barrier against genomic instability.
    Thematic Areas: Neuroscience (miscellaneous) Neuroscience (all) Molecular biology Medicine (miscellaneous) Medicina ii Immunology and microbiology (miscellaneous) Immunology and microbiology (all) General neuroscience General immunology and microbiology General biochemistry,genetics and molecular biology Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Biochemistry & molecular biology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: oscar.yanes@urv.cat
    Author identifier: 0000-0003-3695-7157
    Record's date: 2023-02-19
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.embopress.org/doi/full/10.15252/embj.2019103838
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Embo Journal. 39 (e103838): e103838-
    APA: Pelletier J., Riaño-Canalias F., Almacellas E., Mauvezin C., Samino S., Feu S., Menoyo S., Domostegui A., Garcia-Cajide M., Salazar R., Cortés C., Mar (2020). Nucleotide depletion reveals the impaired ribosome biogenesis checkpoint as a barrier against DNA damage. Embo Journal, 39(e103838), e103838-. DOI: 10.15252/embj.2019103838
    Article's DOI: 10.15252/embj.2019103838
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Biochemistry, Genetics and Molecular Biology (Miscellaneous),Cell Biology,Immunology and Microbiology (Miscellaneous),Medicine (Miscellaneous),Molecular Biology,Neuroscience (Miscellaneous)
    Suppression
    S-phase
    Rna-synthesis
    Proliferation
    P53
    P21
    Nucleotides
    Myc
    Metabolism
    Irbc
    Impdh
    Dna-damage
    Cell-cycle arrest
    Cancer
    Activation
    p21
    nucleotides
    impdh
    irbc
    Neuroscience (miscellaneous)
    Neuroscience (all)
    Molecular biology
    Medicine (miscellaneous)
    Medicina ii
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    General neuroscience
    General immunology and microbiology
    General biochemistry,genetics and molecular biology
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biotecnología
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
    Biochemistry & molecular biology
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