Articles producció científica> Medicina i Cirurgia

Application of Dried Blood Spot Cards combined with liquid chromatography-tandem mass spectrometry to determine eight fat-soluble micronutrients in human blood

  • Identification data

    Identifier: imarina:8679862
    Authors:
    Rubio, LauraYuste, SilviaLudwig, IziarRomero, Maria-PazMotilva, Maria-JoseCalderon, LorenaPla-Paga, LauraCompanys, JuditMacia, Alba
    Abstract:
    The analysis of the fat-soluble vitamins A and E and lipid micronutrients in blood, such as carotenoids, is an important parameter to monitor the micronutrient status in humans. Although the potential of dried blood spot (DBS) cards, the use of this technique for blood sampling and subsequent analysis of these fat-soluble micro nutrients has been poorly or not studied. An analytical method based on DBS cards (FTA (R) DMPK-A) combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed and validated for the determination of carotenoids (lutein, zeaxanthin, beta-cryptoxanthin and beta-carotene), tocopherols (alpha-tocopherol, gamma-tocopherol and delta-tocopherol) and all-trans-retinol in human blood. Under optimum DBS card extraction conditions, the extraction recoveries of the studied compounds were higher than 72%, the sample matrix effect lower than 17%, and the detection limits at hundred nM concentration levels. The developed method was applied to the analysis of human blood, and the concentration ranges obtained fell within the expected ranges previously reported in healthy adults. Moreover, the influence of hematocrit effect was investigated in a range of 25-55% in order to compare the obtained results to those reported in the literature for the analysis of plasma samples. This method represents an improvement over current techniques reported in the literature due to the use of a non-invasive blood collection method, and moreover, this methodology was for the first time 1) validated for the analysis of all-trans-retinol, tocopherols and carotenoids, and 2) applied for the determination of tocopherols in human blood samples.
  • Others:

    Author, as appears in the article.: Rubio, Laura; Yuste, Silvia; Ludwig, Iziar; Romero, Maria-Paz; Motilva, Maria-Jose; Calderon, Lorena; Pla-Paga, Laura; Companys, Judit; Macia, Alba;
    Department: Medicina i Cirurgia
    URV's Author/s: Calderon Pérez, Lorena / Companys Alemany, Judit / PLA PAGA, LAURA
    Keywords: Whole blood Vitamin-a Tocopherols Tandem ms Samples Retinol Population Plasma Phenols Hydroxytyrosol metabolites Dbs cards Carotenoids Beta-carotene
    Abstract: The analysis of the fat-soluble vitamins A and E and lipid micronutrients in blood, such as carotenoids, is an important parameter to monitor the micronutrient status in humans. Although the potential of dried blood spot (DBS) cards, the use of this technique for blood sampling and subsequent analysis of these fat-soluble micro nutrients has been poorly or not studied. An analytical method based on DBS cards (FTA (R) DMPK-A) combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed and validated for the determination of carotenoids (lutein, zeaxanthin, beta-cryptoxanthin and beta-carotene), tocopherols (alpha-tocopherol, gamma-tocopherol and delta-tocopherol) and all-trans-retinol in human blood. Under optimum DBS card extraction conditions, the extraction recoveries of the studied compounds were higher than 72%, the sample matrix effect lower than 17%, and the detection limits at hundred nM concentration levels. The developed method was applied to the analysis of human blood, and the concentration ranges obtained fell within the expected ranges previously reported in healthy adults. Moreover, the influence of hematocrit effect was investigated in a range of 25-55% in order to compare the obtained results to those reported in the literature for the analysis of plasma samples. This method represents an improvement over current techniques reported in the literature due to the use of a non-invasive blood collection method, and moreover, this methodology was for the first time 1) validated for the analysis of all-trans-retinol, tocopherols and carotenoids, and 2) applied for the determination of tocopherols in human blood samples.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Engenharias iii Engenharias ii Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, analytical Cell biology Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física Analytical chemistry
    Author's mail: judit.companys@urv.cat lorena.calderon@urv.cat lorena.calderon@urv.cat judit.companys@urv.cat judit.companys@urv.cat
    Author identifier: 0000-0003-1485-0818 0000-0003-0766-0733 0000-0003-0766-0733 0000-0003-1485-0818 0000-0003-1485-0818
    Record's date: 2023-02-23
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1570023220303846?via%3Dihub#!
    Papper original source: Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences. 1152 (122247):
    APA: Rubio, Laura; Yuste, Silvia; Ludwig, Iziar; Romero, Maria-Paz; Motilva, Maria-Jose; Calderon, Lorena; Pla-Paga, Laura; Companys, Judit; Macia, Alba; (2020). Application of Dried Blood Spot Cards combined with liquid chromatography-tandem mass spectrometry to determine eight fat-soluble micronutrients in human blood. Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 1152(122247), -. DOI: 10.1016/j.jchromb.2020.122247
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.jchromb.2020.122247
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Biochemical Research Methods,Biochemistry,Cell Biology,Chemistry, Analytical,Clinical Biochemistry,Medicine (Miscellaneous)
    Whole blood
    Vitamin-a
    Tocopherols
    Tandem ms
    Samples
    Retinol
    Population
    Plasma
    Phenols
    Hydroxytyrosol metabolites
    Dbs cards
    Carotenoids
    Beta-carotene
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General medicine
    Farmacia
    Engenharias iii
    Engenharias ii
    Educação física
    Clinical biochemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, analytical
    Cell biology
    Biotecnología
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Astronomia / física
    Analytical chemistry
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