Articles producció científica> Química Analítica i Química Orgànica

Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry

  • Identification data

    Identifier: imarina:9139012
    Authors:
    Pérez-Alcaraz ABorrull FAguilar CCalull MBenavente F
    Abstract:
    © 2020 Elsevier B.V. This study presents for the first time an in-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method for the enantiodetermination of drugs of abuse in urine samples. The enantioseparation of R,S-3,4-methylenedioxypyrovalerone (R,S-MDPV) was achieved with a 10 mM ammonium acetate BGE (pH 7) that contained 0.5% (m/v) of sulphated-α-CD as chiral selector. At these pH conditions, this CD was negatively charged, which prevented its entrance into the mass spectrometer since it migrates in the opposite direction. To improve sensitivity, an in-line SPE-CE-MS method using high pressure for sample introduction (i.e. 20 min at 3 bars) was developed. Furthermore, the conditioning procedure and the first part of the electrophoretic separation were performed by switching off the nebulizer gas and the ionization source voltage to avoid non-volatile contaminant arrival into the mass spectrometer. The developed methodology was validated by analyzing urine samples, which required a very simple liquid-liquid extraction (LLE) sample pretreatment. Linearity ranged from 30 to 250 ng mL−1, limit of detection (LOD) was 10 ng mL−1, relative standard deviation (RSD) values were below 10.5% in terms of intra-day and inter-day precision and the relative error values were below 9% for peak areas accuracy.
  • Others:

    Author, as appears in the article.: Pérez-Alcaraz A; Borrull F; Aguilar C; Calull M; Benavente F
    Department: Química Analítica i Química Orgànica
    URV's Author/s: Aguilar Anguera, Maria del Carmen / Borrull Ballarín, Francesc / Calull Blanch, Marta
    Keywords: Uv Urine analysis Synthetic cathinones Sulphated cyclodextrins Separation Resolution Methylenedioxypyrovalerone mdpv In-line preconcentration Enantiodetermination in-line preconcentration Enantiodetermination Dopamine Derivatives Cyclodextrin Cathinones Capillary electrophoresis mass spectrometry Amphetamine-type stimulants
    Abstract: © 2020 Elsevier B.V. This study presents for the first time an in-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method for the enantiodetermination of drugs of abuse in urine samples. The enantioseparation of R,S-3,4-methylenedioxypyrovalerone (R,S-MDPV) was achieved with a 10 mM ammonium acetate BGE (pH 7) that contained 0.5% (m/v) of sulphated-α-CD as chiral selector. At these pH conditions, this CD was negatively charged, which prevented its entrance into the mass spectrometer since it migrates in the opposite direction. To improve sensitivity, an in-line SPE-CE-MS method using high pressure for sample introduction (i.e. 20 min at 3 bars) was developed. Furthermore, the conditioning procedure and the first part of the electrophoretic separation were performed by switching off the nebulizer gas and the ionization source voltage to avoid non-volatile contaminant arrival into the mass spectrometer. The developed methodology was validated by analyzing urine samples, which required a very simple liquid-liquid extraction (LLE) sample pretreatment. Linearity ranged from 30 to 250 ng mL−1, limit of detection (LOD) was 10 ng mL−1, relative standard deviation (RSD) values were below 10.5% in terms of intra-day and inter-day precision and the relative error values were below 9% for peak areas accuracy.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine General chemistry Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, analytical Chemistry (miscellaneous) Biotecnología Biodiversidade Biochemistry Astronomia / física Analytical chemistry Administração pública e de empresas, ciências contábeis e turismo
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0039-9140
    Author's mail: carme.aguilar@urv.cat marta.calull@urv.cat francesc.borrull@urv.cat
    Author identifier: 0000-0002-5913-2599 0000-0002-5258-1949 0000-0003-2718-9336
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0039914020312856?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Talanta. 225 (121994): 121994-
    APA: Pérez-Alcaraz A; Borrull F; Aguilar C; Calull M; Benavente F (2021). Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry. Talanta, 225(121994), 121994-. DOI: 10.1016/j.talanta.2020.121994
    Article's DOI: 10.1016/j.talanta.2020.121994
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Biochemistry,Chemistry (Miscellaneous),Chemistry, Analytical,Spectroscopy
    Uv
    Urine analysis
    Synthetic cathinones
    Sulphated cyclodextrins
    Separation
    Resolution
    Methylenedioxypyrovalerone mdpv
    In-line preconcentration
    Enantiodetermination in-line preconcentration
    Enantiodetermination
    Dopamine
    Derivatives
    Cyclodextrin
    Cathinones
    Capillary electrophoresis mass spectrometry
    Amphetamine-type stimulants
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    General chemistry
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, analytical
    Chemistry (miscellaneous)
    Biotecnología
    Biodiversidade
    Biochemistry
    Astronomia / física
    Analytical chemistry
    Administração pública e de empresas, ciências contábeis e turismo
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