Author, as appears in the article.: Castro de Moura, Manuel; Davalos, Veronica; Planas-Serra, Laura; Alvarez-Errico, Damiana; Arribas, Carles; Ruiz, Montserrat; Aguilera-Albesa, Sergio; Troya, Jesus; Valencia-Ramos, Juan; Velez-Santamaria, Valentina; Rodriguez-Palmero, Agusti; Villar-Garcia, Judit; Horcajada, Juan P.; Albu, Sergiu; Casasnovas, Carlos; Rull, Anna; Reverte, Laia; Dietl, Beatriz; Dalmau, David; Arranz, Maria J.; Llucia-Carol, Laia; Planas, Anna M.; Perez-Tur, Jordi; Fernandez-Cadenas, Israel; Villares, Paula; Tenorio, Jair; Colobran, Roger; Martin-Nalda, Andrea; Soler-Palacin, Pere; Vidal, Francesc; Pujol, Aurora; Esteller, Manel;

Department: Medicina i Cirurgia

URV's Author/s: RULL AIXA, ANNA / Vidal Marsal, Francisco

Keywords: Young adult Virus infection Virology Validation study Spain Severity of illness index Severe acute respiratory syndrome coronavirus 2 Sars-cov-2 Respiratory insufficiency Respiratory failure Reproducibility of results Reproducibility Pyrosequencing Pneumonia Oxygen therapy Noninvasive ventilation Middle aged Mgmt Metabolism Melanoma Male Major histocompatibility antigen class 1 Major clinical study Interferons Interferon Intensive care unit Inflammasome Humans Human Hospitalization Heredity Genome-wide association study Genetics Female Extracorporeal oxygenation Etiology Epigenome Epigenetics Dna methylation Disease severity Cross validation Cpg islands Cpg island Covid-19 Coronavirus disease 2019 Coronavirus Cohort studies Cohort analysis Clinical outcome Chromosome Blood sampling Biological marker Bioinformatics Assisted ventilation Artificial ventilation Article Adult

Abstract: Background: Patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the coronavirus disease 2019 (COVID-19), exhibit a wide spectrum of disease behaviour. Since DNA methylation has been implicated in the regulation of viral infections and the immune system, we performed an epigenome-wide association study (EWAS) to identify candidate loci regulated by this epigenetic mark that could be involved in the onset of COVID-19 in patients without comorbidities. Methods: Peripheral blood samples were obtained from 407 confirmed COVID-19 patients <= 61 years of age and without comorbidities, 194 (47.7%) of whom had mild symptomatology that did not involve hospitaliza-tion and 213 (52.3%) had a severe clinical course that required respiratory support. The set of cases was divided into discovery (n = 207) and validation (n = 200) cohorts, balanced for age and sex of individuals. We analysed the DNA methylation status of 850,000 CpG sites in these patients. Findings: The DNA methylation status of 44 CpG sites was associated with the clinical severity of COVID-19. Of these loci, 23 (52.3%) were located in 20 annotated coding genes. These genes, such as the inflammasome component Absent in Melanoma 2 (AIM2) and the Major Histocompatibility Complex, class I C (HLA-C) candi-dates, were mainly involved in the response of interferon to viral infection. We used the EWAS-identified sites to establish a DNA methylation signature (EPICOVID) that is associated with the severity of the disease. Interpretation: We identified DNA methylation sites as epigenetic susceptibility loci for respiratory failure in COVID-19 patients. These candidate biomarkers, combined with other clinical, cellular and genetic factors, could be useful in the clinical stratification and management of patients infected with the SARS-CoV-2. (C) 2021 The Authors. Published by Elsevier B.V.

Thematic Areas: Saúde coletiva Medicine, research & experimental Medicine (miscellaneous) Medicina iii Medicina ii Medicina i General medicine General biochemistry,genetics and molecular biology Ciências biológicas ii Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2352-3964

Author's mail: francesc.vidal@urv.cat

Author identifier: 0000-0002-6692-6186

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.thelancet.com/journals/ebiom/article/PIIS2352-39642100132-8/fulltext

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Ebiomedicine. 66 (103339):

APA: Castro de Moura, Manuel; Davalos, Veronica; Planas-Serra, Laura; Alvarez-Errico, Damiana; Arribas, Carles; Ruiz, Montserrat; Aguilera-Albesa, Sergio; (2021). Epigenome-wide association study of COVID-19 severity with respiratory failure. Ebiomedicine, 66(103339), -. DOI: 10.1016/j.ebiom.2021.103339

Article's DOI: 10.1016/j.ebiom.2021.103339

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications