Articles producció científica> Medicina i Cirurgia

No evidence for a placental microbiome in human pregnancies at term

  • Identification data

    Identifier: imarina:9216884
    Authors:
    Sterpu, IreneFransson, EmmaHugerth, Luisa W.Du, JuanPereira, MarcelaCheng, LiqinRadu, Sebastian AlexandruCalderon-Perez, LorenaZha, YinghuaAngelidou, PiaPennhag, AlexandraBoulund, FredrikScheynius, AnnikaEngstrand, LarsWiberg-Itzel, EvaSchuppe-Koistinen, Ina
    Abstract:
    BACKGROUND: The placenta plays an important role in the modulation of pregnancy immunity; however, there is no consensus regarding the existence of a placental microbiome in healthy full-term pregnancies. OBJECTIVE: This study aimed to investigate the existence and origin of a placental microbiome. STUDY DESIGN: A cross-sectional study comparing samples (3 layers of placental tissue, amniotic fluid, vernix caseosa, and saliva, vaginal, and rectal samples) from 2 groups of full-term births: 50 women not in labor with elective cesarean deliveries and 26 with vaginal deliveries. The comparisons were performed using polymerase chain reaction amplification and DNA sequencing techniques and bacterial culture experiments. RESULTS: There were no significant differences regarding background characteristics between women who delivered by elective cesarean and those who delivered vaginally. Quantitative measurements of bacterial content in all 3 placental layers (quantitative polymerase chain reaction of the 16S ribosomal RNA gene) did not show any significant difference among any of the sample types and the negative controls. Here, 16S ribosomal RNA gene sequencing of the maternal side of the placenta could not differentiate between bacteria in the placental tissue and contamination of the laboratory reagents with bacterial DNA. Probe-specific quantitative polymerase chain reaction for bacterial taxa suspected to be present in the placenta could not detect any statistically significant difference between the 2 groups. In bacterial cultures, substantially more bacteria were observed in the placenta layers from vaginal deliveries than those from cesarean deliveries. In addition, 16S ribosomal RNA gene sequencing of bacterial colonies revealed that most of the bacteria that grew on
  • Others:

    Author, as appears in the article.: Sterpu, Irene; Fransson, Emma; Hugerth, Luisa W.; Du, Juan; Pereira, Marcela; Cheng, Liqin; Radu, Sebastian Alexandru; Calderon-Perez, Lorena; Zha, Yinghua; Angelidou, Pia; Pennhag, Alexandra; Boulund, Fredrik; Scheynius, Annika; Engstrand, Lars; Wiberg-Itzel, Eva; Schuppe-Koistinen, Ina;
    Department: Medicina i Cirurgia
    URV's Author/s: Calderon Pérez, Lorena
    Keywords: Vernix caseosa Vaginal fluid Saliva Quantitative polymerase chain reaction Pregnancy Placenta Microbiome In utero colonization Feces Contamination Bacterial culture Amniotic fluid 16s rna gene
    Abstract: BACKGROUND: The placenta plays an important role in the modulation of pregnancy immunity; however, there is no consensus regarding the existence of a placental microbiome in healthy full-term pregnancies. OBJECTIVE: This study aimed to investigate the existence and origin of a placental microbiome. STUDY DESIGN: A cross-sectional study comparing samples (3 layers of placental tissue, amniotic fluid, vernix caseosa, and saliva, vaginal, and rectal samples) from 2 groups of full-term births: 50 women not in labor with elective cesarean deliveries and 26 with vaginal deliveries. The comparisons were performed using polymerase chain reaction amplification and DNA sequencing techniques and bacterial culture experiments. RESULTS: There were no significant differences regarding background characteristics between women who delivered by elective cesarean and those who delivered vaginally. Quantitative measurements of bacterial content in all 3 placental layers (quantitative polymerase chain reaction of the 16S ribosomal RNA gene) did not show any significant difference among any of the sample types and the negative controls. Here, 16S ribosomal RNA gene sequencing of the maternal side of the placenta could not differentiate between bacteria in the placental tissue and contamination of the laboratory reagents with bacterial DNA. Probe-specific quantitative polymerase chain reaction for bacterial taxa suspected to be present in the placenta could not detect any statistically significant difference between the 2 groups. In bacterial cultures, substantially more bacteria were observed in the placenta layers from vaginal deliveries than those from cesarean deliveries. In addition, 16S ribosomal RNA gene sequencing of bacterial colonies revealed that most of the bacteria that grew on the plates were genera typically found in human skin; moreover, it revealed that placentas delivered vaginally contained a high prevalence of common vaginal bacteria. Bacterial growth inhibition experiments indicated that placental tissue may facilitate the inhibition of bacterial growth. CONCLUSION: We found no evidence to support the existence of a placental microbiome in our study of 76 term pregnancies, which used polymerase chain reaction amplification and sequencing techniques and bacterial culture experiments. Incidental findings of bacterial species could be due to contamination or to low-grade bacterial presence in some locations; such bacteria do not represent a placental microbiome per se.
    Thematic Areas: Saúde coletiva Odontología Obstetrics and gynecology Obstetrics & gynecology Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Ciências biológicas ii Ciências biológicas i Biotecnología Biodiversidade
    Author's mail: lorena.calderon@urv.cat lorena.calderon@urv.cat
    Author identifier: 0000-0003-0766-0733 0000-0003-0766-0733
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.ajog.org/article/S0002-9378(20)30984-4/fulltext
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: American Journal Of Obstetrics And Gynecology. 224 (3):
    APA: Sterpu, Irene; Fransson, Emma; Hugerth, Luisa W.; Du, Juan; Pereira, Marcela; Cheng, Liqin; Radu, Sebastian Alexandru; Calderon-Perez, Lorena; Zha, Yi (2021). No evidence for a placental microbiome in human pregnancies at term. American Journal Of Obstetrics And Gynecology, 224(3), -. DOI: 10.1016/j.ajog.2020.08.103
    Article's DOI: 10.1016/j.ajog.2020.08.103
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Obstetrics & Gynecology,Obstetrics and Gynecology
    Vernix caseosa
    Vaginal fluid
    Saliva
    Quantitative polymerase chain reaction
    Pregnancy
    Placenta
    Microbiome
    In utero colonization
    Feces
    Contamination
    Bacterial culture
    Amniotic fluid
    16s rna gene
    Saúde coletiva
    Odontología
    Obstetrics and gynecology
    Obstetrics & gynecology
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Farmacia
    Engenharias iv
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biodiversidade
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