Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses

  • Identification data

    Identifier: imarina:9242255
    Authors:
    Dabare PRLBachhuka AParkinson-Lawrence EVasilev K
    Abstract:
    Surface roughness plays an important role in regulating protein adsorption to biomaterial surfaces and modulating the subsequent inflammatory response. In this study, we examined the role of surface nanotopography on albumin adsorption, unfolding and subsequent immune responses. To achieve the objectives of the study, we create model surfaces of hill-like nanoprotrusions by covalently immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, and 68 nm) on a functional plasma polymer layer. The amount of adsorbed albumin increased with the increase in surface area caused by greater surface nanotopography scales. Circular dichroism spectroscopy was used to evaluate albumin conformational changes and pointed to loss of ?-helical structure on all model surfaces with the greatest conformational changes found on the smooth surface and the surface with largest nanotopography features. Studies with differentiated THP-1 cells (dTHP-1) demonstrated that immune cells interacted with surface adsorbed albumin via their scavenger receptors, which could bind to exposed peptide sequences caused by surface induced unfolding of the albumin. Pre-adsorption of albumin resulted in an overall decrease in the level of expression of pro-inflammatory cytokines from dTHP-1 cells. On the other hand, pre-adsorption of albumin led in an increase in the production of anti-inflammatory markers, which suggests a switch to the M2 pro-healing phenotype. The knowledge obtained from this study could instruct the design of healthcare materials where the generation of targeted surface nanotopography and pre-adsorption of albumin may enhance the biomaterial biocompatibility and lead to faster wound healing. © 2021 The Authors
  • Others:

    Author, as appears in the article.: Dabare PRL; Bachhuka A; Parkinson-Lawrence E; Vasilev K
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Bachhuka, Akash
    Keywords: Unfoldings Surface roughness Surface nanotopography Proteins Pre-adsorption Plasma polymerization Nanotopography Nanotopographies Model surface Immune system Immune response Immune cells interaction Immune cells Immune cell interaction Gold nanoparticles Dichroism Cytology Circular dichroism spectroscopy Cells Cell interaction Biocompatibility Albumin adsorption Adsorption
    Abstract: Surface roughness plays an important role in regulating protein adsorption to biomaterial surfaces and modulating the subsequent inflammatory response. In this study, we examined the role of surface nanotopography on albumin adsorption, unfolding and subsequent immune responses. To achieve the objectives of the study, we create model surfaces of hill-like nanoprotrusions by covalently immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, and 68 nm) on a functional plasma polymer layer. The amount of adsorbed albumin increased with the increase in surface area caused by greater surface nanotopography scales. Circular dichroism spectroscopy was used to evaluate albumin conformational changes and pointed to loss of ?-helical structure on all model surfaces with the greatest conformational changes found on the smooth surface and the surface with largest nanotopography features. Studies with differentiated THP-1 cells (dTHP-1) demonstrated that immune cells interacted with surface adsorbed albumin via their scavenger receptors, which could bind to exposed peptide sequences caused by surface induced unfolding of the albumin. Pre-adsorption of albumin resulted in an overall decrease in the level of expression of pro-inflammatory cytokines from dTHP-1 cells. On the other hand, pre-adsorption of albumin led in an increase in the production of anti-inflammatory markers, which suggests a switch to the M2 pro-healing phenotype. The knowledge obtained from this study could instruct the design of healthcare materials where the generation of targeted surface nanotopography and pre-adsorption of albumin may enhance the biomaterial biocompatibility and lead to faster wound healing. © 2021 The Authors
    Thematic Areas: Mechanical engineering Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: akash.bachhuka@urv.cat
    Author identifier: 0000-0003-1253-8126
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S2590049821000576?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Materials Today Advances. 12
    APA: Dabare PRL; Bachhuka A; Parkinson-Lawrence E; Vasilev K (2021). Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses. Materials Today Advances, 12(), -. DOI: 10.1016/j.mtadv.2021.100187
    Article's DOI: 10.1016/j.mtadv.2021.100187
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Mechanical Engineering
    Unfoldings
    Surface roughness
    Surface nanotopography
    Proteins
    Pre-adsorption
    Plasma polymerization
    Nanotopography
    Nanotopographies
    Model surface
    Immune system
    Immune response
    Immune cells interaction
    Immune cells
    Immune cell interaction
    Gold nanoparticles
    Dichroism
    Cytology
    Circular dichroism spectroscopy
    Cells
    Cell interaction
    Biocompatibility
    Albumin adsorption
    Adsorption
    Mechanical engineering
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
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