Articles producció científica> Bioquímica i Biotecnologia

Differentiation of Saccharomyces species by lipid and metabolome profiles from a single colony

  • Identification data

    Identifier: imarina:9242316
    Authors:
    Ruiz-de-Villa CPoblet MBordons AReguant CRozès N
    Abstract:
    Yeast metabolism depends on growing conditions, which include the chemical composition of the medium, temperature and growth time. Historically, fatty acid profiles have been used to differentiate yeasts growing in liquid media. The present study determined the fatty acids of Saccharomyces species in colonies. Using the same method, the effect of that the number of colonies and growth time had on solid media allowed us to determine the metabolomic profiles of the cells. Our results showed that the lipid and metabolomic profiles of the cells evolved as the colony grew. Interestingly, some strains of Saccharomyces cerevisiae have been were differentiated using the fatty acid profile of a colony; concretely indeed EC1118 and QA23 strains were separated from ICV-K1 and BM4x4. The synthesis of saturated fatty acids was greater than that of unsaturated fatty acids during the first two days of cell growth on a solid medium compared to a liquid medium. Unsaturated fatty acids subsequently became predominant. Finally, this methodology could be useful for carrying out physiological studies in a complete or defined solid growth medium allowing the supplementation of compounds, which inhibit or activate the growth of yeasts. © 2021
  • Others:

    Author, as appears in the article.: Ruiz-de-Villa C; Poblet M; Bordons A; Reguant C; Rozès N
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Bordons de Porrata- Doria, Albert / Poblet Icart, Maria Montserrat / Reguant Miranda, Cristina / Rozès, Nicolas Andre Louis / Ruiz De villa sardón, Candela
    Keywords: Wine yeast Trehalose Squalene Saccharomyces cerevisiae S. uvarum S. kudriavzevii Fatty acids
    Abstract: Yeast metabolism depends on growing conditions, which include the chemical composition of the medium, temperature and growth time. Historically, fatty acid profiles have been used to differentiate yeasts growing in liquid media. The present study determined the fatty acids of Saccharomyces species in colonies. Using the same method, the effect of that the number of colonies and growth time had on solid media allowed us to determine the metabolomic profiles of the cells. Our results showed that the lipid and metabolomic profiles of the cells evolved as the colony grew. Interestingly, some strains of Saccharomyces cerevisiae have been were differentiated using the fatty acid profile of a colony; concretely indeed EC1118 and QA23 strains were separated from ICV-K1 and BM4x4. The synthesis of saturated fatty acids was greater than that of unsaturated fatty acids during the first two days of cell growth on a solid medium compared to a liquid medium. Unsaturated fatty acids subsequently became predominant. Finally, this methodology could be useful for carrying out physiological studies in a complete or defined solid growth medium allowing the supplementation of compounds, which inhibit or activate the growth of yeasts. © 2021
    Thematic Areas: Zootecnia / recursos pesqueiros Química Nutrição Microbiology Medicina veterinaria Medicina i Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: candela.ruiz@urv.cat nicolasrozes@urv.cat albert.bordons@urv.cat cristina.reguant@urv.cat montserrat.poblet@urv.cat
    Author identifier: 0000-0001-9718-3429 0000-0002-5320-8740 0000-0002-5036-1408
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0740002021002306
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Food Microbiology. 103 103964-
    APA: Ruiz-de-Villa C; Poblet M; Bordons A; Reguant C; Rozès N (2022). Differentiation of Saccharomyces species by lipid and metabolome profiles from a single colony. Food Microbiology, 103(), 103964-. DOI: 10.1016/j.fm.2021.103964
    Article's DOI: 10.1016/j.fm.2021.103964
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Biotechnology & Applied Microbiology,Food Science,Food Science & Technology,Microbiology
    Wine yeast
    Trehalose
    Squalene
    Saccharomyces cerevisiae
    S. uvarum
    S. kudriavzevii
    Fatty acids
    Zootecnia / recursos pesqueiros
    Química
    Nutrição
    Microbiology
    Medicina veterinaria
    Medicina i
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology & applied microbiology
    Biodiversidade
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