Author, as appears in the article.: Ruiz-de-Villa C; Poblet M; Bordons A; Reguant C; Rozès N
Department: Bioquímica i Biotecnologia
URV's Author/s: Bordons de Porrata- Doria, Albert / Poblet Icart, Maria Montserrat / Reguant Miranda, Cristina / Rozès, Nicolas Andre Louis / Ruiz De villa sardón, Candela
Keywords: Wine yeast Trehalose Squalene Saccharomyces cerevisiae S. uvarum S. kudriavzevii Fatty acids
Abstract: Yeast metabolism depends on growing conditions, which include the chemical composition of the medium, temperature and growth time. Historically, fatty acid profiles have been used to differentiate yeasts growing in liquid media. The present study determined the fatty acids of Saccharomyces species in colonies. Using the same method, the effect of that the number of colonies and growth time had on solid media allowed us to determine the metabolomic profiles of the cells. Our results showed that the lipid and metabolomic profiles of the cells evolved as the colony grew. Interestingly, some strains of Saccharomyces cerevisiae have been were differentiated using the fatty acid profile of a colony; concretely indeed EC1118 and QA23 strains were separated from ICV-K1 and BM4x4. The synthesis of saturated fatty acids was greater than that of unsaturated fatty acids during the first two days of cell growth on a solid medium compared to a liquid medium. Unsaturated fatty acids subsequently became predominant. Finally, this methodology could be useful for carrying out physiological studies in a complete or defined solid growth medium allowing the supplementation of compounds, which inhibit or activate the growth of yeasts. © 2021
Thematic Areas: Zootecnia / recursos pesqueiros Química Nutrição Microbiology Medicina veterinaria Medicina i Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: candela.ruiz@urv.cat nicolasrozes@urv.cat albert.bordons@urv.cat cristina.reguant@urv.cat montserrat.poblet@urv.cat
Author identifier: 0000-0001-9718-3429 0000-0002-5320-8740 0000-0002-5036-1408
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0740002021002306
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Food Microbiology. 103 103964-
APA: Ruiz-de-Villa C; Poblet M; Bordons A; Reguant C; Rozès N (2022). Differentiation of Saccharomyces species by lipid and metabolome profiles from a single colony. Food Microbiology, 103(), 103964-. DOI: 10.1016/j.fm.2021.103964
Article's DOI: 10.1016/j.fm.2021.103964
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications