Articles producció científica> Bioquímica i Biotecnologia

GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon

  • Identification data

    Identifier: imarina:9249027
    Authors:
    Grau-Bove, CarmeGonzalez-Quilen, CarlosCantini, GiuliaNardini, PatriziaEspina, BeatrizBani, DanieleTerra, XimenaBlay, MTeresaRodriguez-Gallego, EstherLuconi, MichaelaArdevol, AnnaPinent, Montserrat
    Abstract:
    GLP1 produced in the upper part of the gut is released after food intake and acts by activat-ing insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine mechanisms of action of these enterohormones in the human colon. We stimulated human colon tissue in different ex vivo models with meat peptone and we used immunofluorescence to study the presence of canonical and non-canonical receptors of GLP1. We found that PYY and GLP1 are secreted mainly at the gut lumen in unstimulated and stimulated conditions. We detected DPP4 activity and found that GLP1R and GCGR are widely expressed in the human colon epithelium. Unlike GLP1R, GCGR is not expressed in the lamina propria, but it is located in the crypts of Lieberkühn. We detected GLP1R expression in human colon cell culture models. We show that the apical secretion of PYY and GLP1 occurs in humans, and we provide evidence that GLP1 has a potential direct paracrine function through the expression of its receptors in the colon epithelium, opening new therapeutic perspectives in the use of enterohormones analogues in metabolic pathologies.
  • Others:

    Author, as appears in the article.: Grau-Bove, Carme; Gonzalez-Quilen, Carlos; Cantini, Giulia; Nardini, Patrizia; Espina, Beatriz; Bani, Daniele; Terra, Ximena; Blay, MTeresa; Rodriguez-Gallego, Esther; Luconi, Michaela; Ardevol, Anna; Pinent, Montserrat
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Ardévol Grau, Anna / Blay Olivé, Maria Teresa / GONZALEZ QUILEN, CARLOS ALBERTO / Grau Bové, Maria Carmen / Pinent Armengol, Montserrat / Rodríguez Gallego, Esther / Terra Barbadora, Ximena
    Keywords: Pyy Intestinal mucosa Insulin secretion Humans Glucagon-like peptide-1 receptor Glucagon-like peptide-1 Glp1 Colon Apical secretion secretion pyy neurons health gut glycemia expression apical secretion actin
    Abstract: GLP1 produced in the upper part of the gut is released after food intake and acts by activat-ing insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine mechanisms of action of these enterohormones in the human colon. We stimulated human colon tissue in different ex vivo models with meat peptone and we used immunofluorescence to study the presence of canonical and non-canonical receptors of GLP1. We found that PYY and GLP1 are secreted mainly at the gut lumen in unstimulated and stimulated conditions. We detected DPP4 activity and found that GLP1R and GCGR are widely expressed in the human colon epithelium. Unlike GLP1R, GCGR is not expressed in the lamina propria, but it is located in the crypts of Lieberkühn. We detected GLP1R expression in human colon cell culture models. We show that the apical secretion of PYY and GLP1 occurs in humans, and we provide evidence that GLP1 has a potential direct paracrine function through the expression of its receptors in the colon epithelium, opening new therapeutic perspectives in the use of enterohormones analogues in metabolic pathologies.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: mariacarmen.grau@urv.cat mariacarmen.grau@urv.cat mariacarmen.grau@urv.cat anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat esther.rodriguez@urv.cat montserrat.pinent@urv.cat
    Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378
    Record's date: 2024-10-19
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/23/7/3523
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 23 (7): 3523-
    APA: Grau-Bove, Carme; Gonzalez-Quilen, Carlos; Cantini, Giulia; Nardini, Patrizia; Espina, Beatriz; Bani, Daniele; Terra, Ximena; Blay, MTeresa; Rodriguez (2022). GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon. International Journal Of Molecular Sciences, 23(7), 3523-. DOI: 10.3390/ijms23073523
    Article's DOI: 10.3390/ijms23073523
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Pyy
    Intestinal mucosa
    Insulin secretion
    Humans
    Glucagon-like peptide-1 receptor
    Glucagon-like peptide-1
    Glp1
    Colon
    Apical secretion
    secretion
    pyy
    neurons
    health
    gut
    glycemia
    expression
    apical secretion
    actin
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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