Author, as appears in the article.: Grau-Bove, Carme; Gonzalez-Quilen, Carlos; Cantini, Giulia; Nardini, Patrizia; Espina, Beatriz; Bani, Daniele; Terra, Ximena; Blay, MTeresa; Rodriguez-Gallego, Esther; Luconi, Michaela; Ardevol, Anna; Pinent, Montserrat
Department: Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Blay Olivé, Maria Teresa / GONZALEZ QUILEN, CARLOS ALBERTO / Grau Bové, Maria Carmen / Pinent Armengol, Montserrat / Rodríguez Gallego, Esther / Terra Barbadora, Ximena
Keywords: Pyy Intestinal mucosa Insulin secretion Humans Glucagon-like peptide-1 receptor Glucagon-like peptide-1 Glp1 Colon Apical secretion secretion pyy neurons health gut glycemia expression apical secretion actin
Abstract: GLP1 produced in the upper part of the gut is released after food intake and acts by activat-ing insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine mechanisms of action of these enterohormones in the human colon. We stimulated human colon tissue in different ex vivo models with meat peptone and we used immunofluorescence to study the presence of canonical and non-canonical receptors of GLP1. We found that PYY and GLP1 are secreted mainly at the gut lumen in unstimulated and stimulated conditions. We detected DPP4 activity and found that GLP1R and GCGR are widely expressed in the human colon epithelium. Unlike GLP1R, GCGR is not expressed in the lamina propria, but it is located in the crypts of Lieberkühn. We detected GLP1R expression in human colon cell culture models. We show that the apical secretion of PYY and GLP1 occurs in humans, and we provide evidence that GLP1 has a potential direct paracrine function through the expression of its receptors in the colon epithelium, opening new therapeutic perspectives in the use of enterohormones analogues in metabolic pathologies.
Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariacarmen.grau@urv.cat mariacarmen.grau@urv.cat mariacarmen.grau@urv.cat anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat esther.rodriguez@urv.cat montserrat.pinent@urv.cat
Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378
Record's date: 2024-10-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/1422-0067/23/7/3523
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: International Journal Of Molecular Sciences. 23 (7): 3523-
APA: Grau-Bove, Carme; Gonzalez-Quilen, Carlos; Cantini, Giulia; Nardini, Patrizia; Espina, Beatriz; Bani, Daniele; Terra, Ximena; Blay, MTeresa; Rodriguez (2022). GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon. International Journal Of Molecular Sciences, 23(7), 3523-. DOI: 10.3390/ijms23073523
Article's DOI: 10.3390/ijms23073523
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications