Author, as appears in the article.: Ramos-Romero S; Ponomarenko J; Amézqueta S; Hereu M; Miralles-Pérez B; Romeu M; Méndez L; Medina I; Torres JL
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Miralles Pérez, Bernat / Romeu Ferran, Marta
Keywords: Inflammation Iminosugar Iminocyclitol Gut microbiota Dietary fiber D-fagomine Body weight profiles polysaccharide utilization obesity inflammation improvement impact iminosugar iminocyclitol gut microbiota glucose fatty-acids d-fagomine
Abstract: The goal of this work is to explore if the changes induced by d-fagomine in the gut microbiota are compatible with its effect on body weight and inflammation markers in rats. Methods: Sprague Dawley rats were fed a standard diet supplemented with d-fagomine (or not, for comparison) for 6 months. The variables measured were body weight, plasma mediators of inflammation (hydroxyeicosatetraenoic acids, leukotriene B4, and IL-6), and the concentration of acetic acid in feces and plasma. The composition and diversities of microbiota in cecal content and feces were estimated using 16S rRNA metabarcoding and high-throughput sequencing. We found that after just 6 weeks of intake d-fagomine significantly reduced body weight gain, increased the plasma acetate concentration, and reduced the plasma concentration of the pro-inflammatory biomarkers' leukotriene B4, interleukin 6 and 12 hydroxyeicosatetraenoic acids. These changes were associated with a significantly increased prevalence of Bacteroides and Prevotella feces and increased Bacteroides, Prevotella, Clostridium, and Dysgonomonas while reducing Anaerofilum, Blautia, and Oribacterium in cecal content. In conclusion, d-fagomine induced changes in the composition and diversity of gut microbiota similar to those elicited by dietary fiber and compatible with its anti-inflammatory and body-weight-reducing effects.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: bernat.miralles@urv.cat marta.romeu@urv.cat bernat.miralles@urv.cat
Author identifier: 0000-0003-1294-7069 0000-0002-2131-1858 0000-0003-1294-7069
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2072-6643/14/21/4656
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Nutrients. 14 (21):
APA: Ramos-Romero S; Ponomarenko J; Amézqueta S; Hereu M; Miralles-Pérez B; Romeu M; Méndez L; Medina I; Torres JL (2022). Fiber-like Action of d-Fagomine on the Gut Microbiota and Body Weight of Healthy Rats. Nutrients, 14(21), -. DOI: 10.3390/nu14214656
Article's DOI: 10.3390/nu14214656
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV