Author, as appears in the article.: Escher, Beate, I; Blanco, Jordi; Caixach, Josep; Cserbik, Dora; Farre, Maria J; Flores, Cintia; Koenig, Maria; Lee, Jungeun; Nyffeler, Jo; Planas, Carles; Redondo-Hasselerharm, Paula E; Rovira, Joaquim; Sanchis, Josep; Schuhmacher, Marta; Villanueva, Cristina M
Department: Enginyeria Química Ciències Mèdiques Bàsiques
URV's Author/s: Blanco Pérez, Jordi / Rovira Solano, Joaquim / Schuhmacher Ansuategui, Marta
Keywords: Water quality Oxidative stress Neurotoxicity Disinfection by-products Bioassay toxicity oxidative stress-response oxidative stress neurotoxicity micropollutants mammalian-cell cytotoxicity health genotoxicity disinfection by-products dibromoacetonitrile chemicals bioassay bioanalytical assessment
Abstract: Location-specific patterns of regulated and non-regulated disinfection byproducts (DBPs) were detected in tap water samples of the Barcelona Metropolitan Area. However, it remains unclear if the detected DBPs together with undetected DPBs and organic micropollutants can lead to mixture effects in drinking water.To evaluate the neurotoxicity, oxidative stress response and cytotoxicity of 42 tap water samples, 6 treated with activated carbon filters, 5 with reverse osmosis and 9 bottled waters. To compare the measured effects of the extracts with the mixture effects predicted from the detected concentrations and the relative effect potencies of the detected DBPs using the mixture model of concentration addition.Mixtures of organic chemicals in water samples were enriched by solid phase extraction and tested for cytotoxicity and neurite outgrowth inhibition in the neuronal cell line SH-SY5Y and for cytotoxicity and oxidative stress response in the AREc32 assay.Unenriched water did not trigger neurotoxicity or cytotoxicity. After up to 500-fold enrichment, few extracts showed cytotoxicity. Disinfected water showed low neurotoxicity at 20- to 300-fold enrichment and oxidative stress response at 8- to 140-fold enrichment. Non-regulated non-volatile DBPs, particularly (brominated) haloacetonitriles dominated the predicted mixture effects of the detected chemicals and predicted effects agreed with the measured effects. By hierarchical clustering we identified strong geographical patterns in the types of DPBs and their association with effects. Activated carbon filters did not show a consistent reduction of effects but domestic reverse osmosis filters decreased the effect to that of bottled water.Bioassays are an important complement to chemical analysis of disinfection by-products (DBPs) in drinking water. Comparison of the measured oxidative stress response and mixture effects predicted from the detected chemicals and their relative effect potencies allowed the identification of the forcing agents for the mixture effects, which differed by location but were mainly non-regulated DBPs. This study demonstrates the relevance of non-regulated DBPs from a toxicological perspective. In vitro bioassays, in particular reporter gene assays for oxidative stress response that integrate different reactive toxicity pathways including genotoxicity, may therefore serve as sum parameters for drinking water quality assessment.© 2023. The Author(s).
Thematic Areas: Toxicology Saúde coletiva Public, environmental & occupational health Public health, environmental and occupational health Pollution Medicina ii Interdisciplinar Farmacia Epidemiology Environmental sciences Enfermagem Ciências biológicas ii Ciências ambientais
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: joaquim.rovira@urv.cat jordi.blanco@urv.cat marta.schuhmacher@urv.cat
Author identifier: 0000-0003-4399-6138 0000-0001-8016-0984 0000-0003-4381-2490
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.nature.com/articles/s41370-023-00566-6
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Exposure Science And Environmental Epidemiology. 34 (1): 126-135
APA: Escher, Beate, I; Blanco, Jordi; Caixach, Josep; Cserbik, Dora; Farre, Maria J; Flores, Cintia; Koenig, Maria; Lee, Jungeun; Nyffeler, Jo; Planas, Car (2024). In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water. Journal Of Exposure Science And Environmental Epidemiology, 34(1), 126-135. DOI: 10.1038/s41370-023-00566-6
Article's DOI: 10.1038/s41370-023-00566-6
Entity: Universitat Rovira i Virgili
Journal publication year: 2024
Publication Type: Journal Publications
Repositori URV