Author, as appears in the article.: Jiménez-Franco, A; Castañé, H; Martínez-Navidad, C; Placed-Gallego, C; Hernández-Aguilera, A; Fernández-Arroyo, S; Samarra, I; Canela-Capdevila, M; Arenas, M; Zorzano, A; Hernández-Alvarez, MI; del Castillo, D; Paris, M; Menendez, JA; Camps, J; Joven, J
Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia
URV's Author/s: Arenas Prat, Meritxell / Camps Andreu, Jorge / Castañé Vilafranca, Helena / Del Castillo Déjardin, Daniel / FERNANDEZ ARROYO, SALVADOR / Jiménez Franco, Andrea / Joven Maried, Jorge / París Sans, Marta
Keywords: Weight loss Polyunsaturated fatty acids Metaflammation Lipoxygenase Cyclooxygenase Bariatric surgery Adipose lipid turnover weight loss polyunsaturated fatty acids meta flammation macrophage polarization lipoxygenase health fatty-acids disease cyclooxygenase bariatric surgery adiponectin activation
Abstract: The relationship between lipid mediators and severe obesity remains unclear. Our study investigates the impact of severe obesity on plasma concentrations of oxylipins and fatty acids and explores the consequences of weight loss.In the clinical trial identifier NCT05554224 study, 116 patients with severe obesity and 63 overweight/obese healthy controls matched for age and sex (≈2:1) provided plasma. To assess the effect of surgically induced weight loss, we requested paired plasma samples from 44 patients undergoing laparoscopic sleeve gastrectomy one year after the procedure. Oxylipins were measured using ultra-high-pressure liquid chromatography coupled to a triple quadrupole mass spectrometer via semi-targeted lipidomics. Cytokines and markers of interorgan crosstalk were measured using enzyme-linked immunosorbent assays.We observed significantly elevated levels of circulating fatty acids and oxylipins in patients with severe obesity compared to their metabolically healthier overweight/obese counterparts. Our findings indicated that sex and liver disease were not confounding factors, but we observed weak correlations in plasma with circulating adipokines, suggesting the influence of adipose tissue. Importantly, while weight loss restored the balance in circulating fatty acids, it did not fully normalize the oxylipin profile. Before surgery, oxylipins derived from lipoxygenase activity, such as 12-HETE, 11-HDoHE, 14-HDoHE, and 12-HEPE, were predominant. However, one year following laparoscopic sleeve gastrectomy, we observed a complex shift in the oxylipin profile, favoring species from the cyclooxygenase pathway, particularly proinflammatory prostanoids like TXB2, PGE2, PGD2, and 12-HHTrE. This transformation appears to be linked to a reduction in adiposity, underscoring the role of lipid turnover in the development of metabolic disorders associated with severe obesity.Despite the reduction in fatty acid levels associated with weight loss, the oxylipin profile shifts towards a predominance of more proinflammatory species. These observations underscore the significance of seeking mechanistic approaches to address severe obesity and emphasize the importance of closely monitoring the metabolic adaptations after weight loss.Copyright © 2023 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: andrea.jimenez@urv.cat marta.paris@urv.cat jorge.camps@urv.cat danieldel.castillo@urv.cat helena.castane@estudiants.urv.cat meritxell.arenas@urv.cat jorge.joven@urv.cat
Author identifier: 0000-0002-3165-3640 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-0815-2570 0000-0003-2749-4541
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Clinical Nutrition. 43 (1): 246-258
APA: Jiménez-Franco, A; Castañé, H; Martínez-Navidad, C; Placed-Gallego, C; Hernández-Aguilera, A; Fernández-Arroyo, S; Samarra, I; Canela-Capdevila, M; Ar (2024). Metabolic adaptations in severe obesity: Insights from circulating oxylipins before and after weight loss. Clinical Nutrition, 43(1), 246-258. DOI: 10.1016/j.clnu.2023.12.002
Entity: Universitat Rovira i Virgili
Journal publication year: 2024
Publication Type: Journal Publications
Repositori URV