Autor según el artículo: Tatiana Baciu; Francesc Borrull; Carme Aguilar; Marta Calull

Departamento: Química Analítica i Química Orgànica

Autor/es de la URV: BACIU ., TATIANA; BORRULL BALLARÍN, FRANCESC; AGUILAR ANGUERA, MARIA DEL CARMEN; CALULL BLANCH, MARTA

Palabras clave: Cyclodextrin drugs of abuse pressurized liquid extraction

Resumen: A suitable method has been developed and validated for simultaneously determining cocaine and its major metabolite, benzoylecgonine, 6-acetylmorphine, codeine, morphine and the methadone enantiomers in human hair samples by the in-line coupling between SPE and CyD-assisted CE with a previous sample pretreatment procedure based on pressurized liquid extraction. Optimal separation was achieved on a fused silica-capillary of 50 μm i.d. and 80 cm total length using 11 mM α-CyD in an aqueous solution of 80 mM sodium phosphate at pH 2.5 as the separation medium and an applied voltage of 30 kV. The SPE-CE device consisted of a short length of capillary packed with Oasis HLB sorbent, which was inserted near to the inlet end of the CE capillary. Several parameters affecting the in-line preconcentration were evaluated. The LOQs reached for hair samples were in the range of 0.3–2.5 ng/mg with satisfactory analytical precision in both intraday and day-to-day experiments (RSDs <13%). Relative recoveries greater than 80% were obtained. The method has successfully been applied to the determination of these drugs of abuse in segmented hair from drug abusers who were undergoing methadone maintenance treatment. The results were consistent with the patients’ statements, indicating that the method established herein can be used for verifying a history of drug abuse.

Grupo de investigación: Grup de Cromatografia. Aplicacions Mediambientals

Áreas temáticas: Química Química Chemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0731-7085

Identificador del autor: n/a; 0000-0003-2718-9336; 0000-0002-5913-2599; 0000-0002-5258-1949

Fecha de alta del registro: 2016-10-10

Página final: 428

Volumen de revista: 131

Versión del articulo depositado: info:eu-repo/semantics/submittedVersion

Enlace a la fuente original: http://www.sciencedirect.com/science/article/pii/S0731708516306057

DOI del artículo: 10.1016/j.jpba.2016.09.017

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 420

Tipo de publicación: Article Artículo Article